Browsing by Author "Goni, Felix M"
Now showing items 21-40 of 50
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Exploring Polar Headgroup Interactions Between Sphingomyelin and Ceramide with Infrared Spectroscopy
De la Arada Echevarría, Igor ; González Ramírez, Emilio José; Alonso Izquierdo, Alicia ; Goñi Urcelay, Félix María ; Rodríguez Arrondo, José Luis (Nature, 2020-10-19)Ceramide is a major actor in the sphingolipid signaling pathway elicited by various kinds of cell stress. Under those conditions ceramide (Cer) is produced in the plasma membrane as a product of sphingomyelin (SM) hydrolysis, ... -
Human ATG3 binding to lipid bilayers: role of lipid geometry, and electric charge
Hervás Hidalgo, Javier ; Landajuela Larma, Ane; Antón Helas, Zuriñe; Shnyrova Zhadan, Anna ; Goñi Urcelay, Félix María ; Alonso Izquierdo, Alicia (Nature Publishing, 2017-11-15)Specific protein-lipid interactions lead to a gradual recruitment of AuTophaGy-related (ATG) proteins to the nascent membrane during autophagosome (AP) formation. ATG3, a key protein in the movement of LC3 towards the ... -
Imaging the early stages of phospholipase C/sphingomyelinase activity on vesicles containing coexisting order-disorder and gel-fluid domains
Ibarguren, Maitane ; López Jiménez, David ; Montes, L. Ruth; Sot, Jesus ; Vasil, Michael L.; Vasil, Adriana I.; Goñi Urcelay, Félix María ; Alonso Izquierdo, Alicia (Elsevier, 2011-04)[EN] The binding and early stages of activity of a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa on giant unilamellar vesicles (GUV) have been monitored using fl uorescence confocal microscopy. Both the ... -
Interaction between colicin A pore-forming domain and its cognate immunity protein
Metola Martínez, Ane (2017-07-14)La colicina A y en concreto el dominio formador de poros, pfColA es expresado por Escherichia coli y resulta tóxico para cepas relacionadas. Las bacterias productoras de la toxina expresan la proteína de inmunidad Cai que ... -
Izozte-urtze ziklo bidezko mintz-ereduen fusioa
Gutiérrez Monasterio, Bingen ; Goñi Urcelay, Félix María (Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2020)Mintz ereduak, bai zelularen, baita zelularen organuluen arkitektura, imita dezaketenez, proteina-lipido elkarrekintzak, geruza bikoitzean txertatutako proteinen egiturak edota mintzen dinamika ikertzeko erabilgarriak izan ... -
LC3 subfamily in cardiolipin-mediated mitophagy: a comparison of the LC3A, LC3B and LC3C homologs
Iriondo Nagore, Marina; Etxaniz Iriondo, Asier; Varela Fernández, Yaiza; Ballesteros Rivero, Uxue; Hervás Hidalgo, Javier ; Montes Burgos, Lidia Ruth ; Goñi Urcelay, Félix María ; Alonso Izquierdo, Alicia (Taylor & Francis, 2022-12)Externalization of the phospholipid cardiolipin (CL) to the outer mitochondrial membrane has been proposed to act as a mitophagy trigger. CL would act as a signal for binding the LC3 macroauto-phagy/autophagy proteins. As ... -
Lipid Self-Assemblies under the Atomic Force Microscope
Lipid model membranes are important tools in the study of biophysical processes such as lipid self-assembly and lipid–lipid interactions in cell membranes. The use of model systems to adequate and modulate complexity helps ... -
Lipid-lipid interactions in bilayers containing polar sphingolipids, ceramide and cholesterol.
González Ramírez, Emilio José (2019-04-11)La presente tesis se ha centrado el estudio de las interacciones lípido-lípido asociadas a la segregación lateral de esfingolipidos y colesterol. Estos estudios se han llevado a cabo utilizando sistemas de membranas modelo ... -
Lipids in Mitochondrial Macroautophagy: Phase Behavior of Bilayers Containing Cardiolipin and Ceramide
Varela Fernández, Yaiza; González Ramírez, Emilio José; Iriondo Nagore, Marina; Ballesteros Rivero, Uxue; Etxaniz Iriondo, Asier; Montes Burgos, Lidia Ruth ; Goñi Urcelay, Félix María ; Alonso Izquierdo, Alicia (MDPI, 2023-03-07)Cardiolipin (CL) is a key lipid for damaged mitochondrial recognition by the LC3/GABARAP human autophagy proteins. The role of ceramide (Cer) in this process is unclear, but CL and Cer have been proposed to coexist in ... -
Liquid-crystalline, liquid-ordered, rippled and gel lipid bilayer phases as observed with nile red fluorescence
Sot, Jesús; Gartzia Rivero, Leire; Bañuelos Prieto, Jorge ; Goñi Urcelay, Félix María ; Alonso Izquierdo, Alicia (Elsevier, 2022-10)The basic matrix of cellular membranes consists of a double layer (bilayer) of phospholipids. Semisynthetic lipid bilayers are commonly used in biophysical studies of membranes. According to tem-perature and composition, ... -
Más sobre los aspectos paracientíficos de la tecnología del DNA recombinante
[ES] Se comienza desmitificando la demonización del consumo de drogas y se sigue que si bien los efectos primarios negativos del consumo constituyen Ja razón legítima de una política preventiva los efectos secundarios ... -
Mecanismos de reconocimiento y control de tráfico endosomal por el complejo retrómero.
Romano Moreno, Miguel (2018-12-10)El retrómero es un complejo multiproteíco que participa en múltiples etapas del tráfico vesicular, controlando el reciclaje de cargos presentes en los endosomas a la membrana plasmática, la red Trans-Golgi, así como a ... -
Mechanical and structural studies of internal lipid-containing bacteriophage PRD1.
Azinas, Stavros (2018-06-18)La protección del genoma viral durante el viaje extracelular es un requisito absoluto para la supervivencia y replicación del virus. Una vez que la partícula viral se une a la célula huésped, puede comenzar la eyección del ... -
Mechanistic details of apoptosis-regulatory proteins BAX and BFL1 at the membrane level
Flores Romero, Héctor (2017-12-18)La homeostasis de tejidos es considerado como el equilibrio entre la muerte y proliferación celular, y es por ello que su desregulación a menudo está asociada a diversas situaciones patológicas, tales como enfermedades ... -
Membrane lipid modifications and therapeutic effects mediated by hydroxydocosahexaenoic acid on Alzheimer's disease
Torres, Manuel; Price, Samantha L.; Fiol-deRoque, Maria Antonia; Marcilla-Etxenike, Amaia; Ahyayauch, Hasna; Barceló-Coblijn, Gwendolyn ; Terés, Silvia; Katsouri, Loukia; Ordinas, Margarita; López Jiménez, David ; Ibarguren, Maitane ; Goñi Urcelay, Félix María ; Busquets, Xavier ; Vitorica Ferrández, Javier; Sastre, Magdalena; Escriba, Pablo (Elsevier, 2013-12-27)[EN] Alzheimer's disease (AD) is a neurodegenerative pathology with relevant unmet therapeutic needs. Both natural aging and AD have been associated with a significant decline in the omega-3 polyunsaturated fatty acid ... -
Membrane properties of ceramide: a fluorescence spectroscopic and microscopic study
Las membranas biológicas son complejas estructuras con una base lipídica y cruciales para lasupervivencia y función de las células. La ceramida es un lípido de membrana con unas característicasfísico-químicas singulares, ... -
Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids
González Ramírez, Emilio José; Goñi Urcelay, Félix María ; Alonso Izquierdo, Alicia (Nature Publishing, 2019-09-16)The properties of bilayers composed of pure brain cerebroside (bCrb) or of binary mixtures of bCrb with brain ceramide, cholesterol, egg phosphatidylcholine or brain sphingomyelin have been studied using a combination of ... -
Papel de la N-nervonil esfingomielina en la organización lateral y la estabilidad nanomecánica de bicapas lipídicas
Areso Zubiaur, Itziar Ibone (2015-06-05)Los lípidos de las membranas biológicas no son perfectamente miscibles entre sí, con frecuencia dan origen a “dominios” bidimensionales separados lateralmente cuando se resuspenden en agua. Las mezclas esfingomielina/dio ... -
Patches and Blebs: A Comparative Study of the Composition and Biophysical Properties of Two Plasma Membrane Preparations from CHO Cells
Gutiérrez Monasterio, Bingen ; Jiménez Rojo, Noemi; García Arribas, Aritz ; Riezman, Howard; Goñi Urcelay, Félix María ; Alonso Izquierdo, Alicia (MDPI, 2020-04-10)This study was aimed at preparing and characterizing plasma membranes (PM) from Chinese Hamster Ovary (CHO) cells. Two methods of PM preparation were applied, one based on adhering cells to a poly-lysine-coated surface, ... -
Pb(II) Induces Scramblase Activation and Ceramide-Domain Generation in Red Blood Cells
Ahyayauch, Hasna; García Arribas, Aritz ; Sot, Jesús; González Ramírez, Emilio José; Busto Gamero, Jon V.; Gutiérrez Monasterio, Bingen ; Jiménez Rojo, Noemi; Contreras Gómez, Xabier ; Rendón Ramírez, Adela; Martín Plágaro, César Augusto; Alonso Izquierdo, Alicia ; Goñi Urcelay, Félix María (Nature Publishing, 2018-05-10)The mechanisms of Pb(II) toxicity have been studied in human red blood cells using confocal microscopy, immunolabeling, fluorescence-activated cell sorting and atomic force microscopy. The process follows a sequence of ...