Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes
dc.contributor.author | Eseberri Barace, Itziar | |
dc.contributor.author | Laurens, Claire | |
dc.contributor.author | Miranda Gómez, Jonatan | |
dc.contributor.author | Louche, Katie | |
dc.contributor.author | Lasa Elguezua, Arrate | |
dc.contributor.author | Moro, Cedric | |
dc.contributor.author | Portillo Baquedano, María Puy | |
dc.date.accessioned | 2021-02-08T11:00:15Z | |
dc.date.available | 2021-02-08T11:00:15Z | |
dc.date.issued | 2021-01-30 | |
dc.identifier | doi: 10.3390/ijms22031384 | |
dc.identifier.citation | International Journal of Molecular Sciences 22 (3) : (2021) // Article ID 1384 | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/10810/50090 | |
dc.description.abstract | henolic compounds have emerged in recent years as an option to face insulin resistance and diabetes. The central aim of this study was: (1) to demonstrate that physiological doses of resveratrol (RSV) or quercetin (Q) can influence glucose metabolism in human myotubes, (2) to establish whether AMP-activated protein kinase (AMPK) and protein kinase B –PKB- (Akt) pathways are involved in this effect. In addition, the effects of these polyphenols on mitochondrial biogenesis and fatty acid oxidation were analysed. Myotubes from healthy donors were cultured for 24 h with either 0.1 μM of RSV or with 10 μM of Q. Glucose metabolism, such as glycogen synthesis, glucose oxidation, and lactate production, were measured with D[U-14C]glucose. β-oxidation using [1–14C]palmitate as well as the expression of key metabolic genes and proteins by Real Time PCR and Western blot were also assessed. Although RSV and Q increased pgc1α expression, they did not significantly change either glucose oxidation or β-oxidation. Q increased AMPK, insulin receptor substrate 1 (IRS-1), and AS160 phosphorylation in basal conditions and glycogen synthase kinase 3 (GSK3β) in insulin-stimulated conditions. RSV tended to increase the phosphorylation rates of AMPK and GSK3β. Both of the polyphenols increased insulin-stimulated glycogen synthesis and reduced lactate production in human myotubes. Thus, physiological doses of RSV or Q may exhibit anti-diabetic actions in human myotubes. | es_ES |
dc.description.sponsorship | This research has been supported by Instituto de Salud Carlos III (CIBERObn) under Grant CB12/03/30007 (01/2013) and by the University of the Basque Country under Grant GIU18-173 (07/2018). | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | resveratrol | es_ES |
dc.subject | quecertin | es_ES |
dc.subject | glucose | es_ES |
dc.subject | human primary myotubes | es_ES |
dc.title | Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-02-05T14:10:11Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/22/3/1384/htm | es_ES |
dc.departamentoes | Farmacia y ciencias de los alimentos | |
dc.departamentoeu | Farmazia eta elikagaien zientziak |
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Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).