An SPM-Enriched Marine Oil Supplement Shifted Microglia Polarization toward M2, Ameliorating Retinal Degeneration in rd10 Mice
dc.contributor.author | Olivares González, Lorena | |
dc.contributor.author | Velasco, Sheyla | |
dc.contributor.author | Gallego Garrido, Idoia | |
dc.contributor.author | Esteban Medina, Marina | |
dc.contributor.author | Puras Ochoa, Gustavo | |
dc.contributor.author | Loucera, Carlos | |
dc.contributor.author | Martínez Romero, Alicia | |
dc.contributor.author | Peña Chilet, María | |
dc.contributor.author | Pedraz Muñoz, José Luis | |
dc.contributor.author | Rodrigo, Regina | |
dc.date.accessioned | 2023-01-24T16:55:32Z | |
dc.date.available | 2023-01-24T16:55:32Z | |
dc.date.issued | 2022-12-30 | |
dc.identifier.citation | Antioxidants 12(1) : (2023) // Article ID 98 | es_ES |
dc.identifier.issn | 2076-3921 | |
dc.identifier.uri | http://hdl.handle.net/10810/59455 | |
dc.description.abstract | Retinitis pigmentosa (RP) is the most common inherited retinal dystrophy causing progressive vision loss. It is accompanied by chronic and sustained inflammation, including M1 microglia activation. This study evaluated the effect of an essential fatty acid (EFA) supplement containing specialized pro-resolving mediators (SPMs), on retinal degeneration and microglia activation in rd10 mice, a model of RP, as well as on LPS-stimulated BV2 cells. The EFA supplement was orally administered to mice from postnatal day (P)9 to P18. At P18, the electrical activity of the retina was examined by electroretinography (ERG) and innate behavior in response to light were measured. Retinal degeneration was studied via histology including the TUNEL assay and microglia immunolabeling. Microglia polarization (M1/M2) was assessed by flow cytometry, qPCR, ELISA and histology. Redox status was analyzed by measuring antioxidant enzymes and markers of oxidative damage. Interestingly, the EFA supplement ameliorated retinal dysfunction and degeneration by improving ERG recording and sensitivity to light, and reducing photoreceptor cell loss. The EFA supplement reduced inflammation and microglia activation attenuating M1 markers as well as inducing a shift to the M2 phenotype in rd10 mouse retinas and LPS-stimulated BV2 cells. It also reduced oxidative stress markers of lipid peroxidation and carbonylation. These findings could open up new therapeutic opportunities based on resolving inflammation with oral supplementation with SPMs such as the EFA supplement. | es_ES |
dc.description.sponsorship | This work was funded by the Spanish Ministry of Economy, Industry, and Competitiveness (MINECO), Carlos III Health Institute (ISCIII), grant numbers PI18/00252, PI20/01305, and co-funded by the European Union—European Regional Development Fund (ERDF) and the European Social Fund (FSE) ‘A way of making Europe’. It was also funded by Junta de Andalucía, grant number PE-0002-2018, CIBERER Cooperative and complementary intramural actions (ACCI) 2018, and RETINA CV. R.R. had an ISCIII Spanish National Health System (SNS) Miguel Servet Type II research contract CP15/00019. S.V. was funded by a Conselleria de Innovación, Universidades, Ciencia y Sociedad, GVA predoctoral contract (ACIF/2021/430). C.L. was funded by a Junta de Andalucía, postdoctoral contract (PAIDI2020- DOC_00350) and co-funded by the FSE 2014–2020. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | inherited retinal dystrophies | es_ES |
dc.subject | retinitis pigmentosa | es_ES |
dc.subject | inflammation | es_ES |
dc.subject | microglia | es_ES |
dc.subject | specialized pro-resolving mediators | es_ES |
dc.subject | oxidative stress | es_ES |
dc.title | An SPM-Enriched Marine Oil Supplement Shifted Microglia Polarization toward M2, Ameliorating Retinal Degeneration in rd10 Mice | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2023-01-20T14:22:40Z | |
dc.rights.holder | © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2076-3921/12/1/98 | es_ES |
dc.identifier.doi | 10.3390/antiox12010098 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | |
dc.departamentoeu | Farmazia eta elikagaien zientziak |
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Except where otherwise noted, this item's license is described as © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).