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dc.contributor.authorDe la Hera Fernández, Omaira
dc.contributor.authorIzaguirre, Aritza
dc.contributor.authorRivas Macho, Arrate
dc.contributor.authorAlonso Rojas, Rosa María ORCID
dc.date.accessioned2024-11-27T13:28:55Z
dc.date.available2024-11-27T13:28:55Z
dc.date.issued2024-11-03
dc.identifier.citationSeparations 11(11) : (2024) // Article ID 317es_ES
dc.identifier.issn2297-8739
dc.identifier.urihttp://hdl.handle.net/10810/70628
dc.description.abstractProtein baits containing fipronil as a biocide have shown their effectivity as a control method for Vespa velutina nigrithorax (Lepeletier, 1836) in apiaries. This biocide is not selective for Vespa velutina, so it is important to use the minimum dose to inactivate a nest. Therefore, the aim of this work was the development of analytical methods for the determination of fipronil in protein baits for quality control purposes and in larvae of Vespa velutina to determine the biocide content after protein bait ingestion and to acquire knowledge on fipronil metabolism in larvae. For this purpose, a Quechers-based HPLC-PDA method was developed and validated for the determination of fipronil in both matrixes. Furthermore, a GC-MS method was developed for the analysis of fipronil and its metabolites in dead Vespa velutina larvae fed with a mash containing 0.01% fipronil. Quechers-based HPLC-DAD allowed for the determination of the fipronil content in baits. Fipronil and the metabolites fipronil sulfone and fipronil sulfide were identified by GC-MS in extracts of larvae fed with a protein mash containing 0.01% fipronil. The transformation of fipronil into fipronil sulfone inside the larvae and the high toxicity of this metabolite open the possibility to produce protein baits with lower biocide concentrations.es_ES
dc.description.sponsorshipOmaira de la Hera thanks the University of the Basque Country (UPV/EHU) for her predoctoral grant. Authors thank the Department of Education of Basque Country Government (projects IT1673-22, PUE 2018_1_0007 and PUE 2021_1_008) and the University of the Basque Country (UPV/EHU) (project US21/35) for financial support.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/es/
dc.subjectQuEChERSes_ES
dc.subjectchromatographyes_ES
dc.subjectfiproniles_ES
dc.subjectprotein bait Vespa velutina larvaes_ES
dc.subjectmetabolismes_ES
dc.titleQuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MSes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2024-11-26T17:43:20Z
dc.rights.holder© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).es_ES
dc.relation.publisherversionhttps://www.mdpi.com/2297-8739/11/11/317es_ES
dc.identifier.doi10.3390/separations11110317
dc.departamentoesQuímica analítica
dc.departamentoeuKimika analitikoa


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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).
Except where otherwise noted, this item's license is described as © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).