QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS
dc.contributor.author | De la Hera Fernández, Omaira | |
dc.contributor.author | Izaguirre, Aritza | |
dc.contributor.author | Rivas Macho, Arrate | |
dc.contributor.author | Alonso Rojas, Rosa María | |
dc.date.accessioned | 2024-11-27T13:28:55Z | |
dc.date.available | 2024-11-27T13:28:55Z | |
dc.date.issued | 2024-11-03 | |
dc.identifier.citation | Separations 11(11) : (2024) // Article ID 317 | es_ES |
dc.identifier.issn | 2297-8739 | |
dc.identifier.uri | http://hdl.handle.net/10810/70628 | |
dc.description.abstract | Protein baits containing fipronil as a biocide have shown their effectivity as a control method for Vespa velutina nigrithorax (Lepeletier, 1836) in apiaries. This biocide is not selective for Vespa velutina, so it is important to use the minimum dose to inactivate a nest. Therefore, the aim of this work was the development of analytical methods for the determination of fipronil in protein baits for quality control purposes and in larvae of Vespa velutina to determine the biocide content after protein bait ingestion and to acquire knowledge on fipronil metabolism in larvae. For this purpose, a Quechers-based HPLC-PDA method was developed and validated for the determination of fipronil in both matrixes. Furthermore, a GC-MS method was developed for the analysis of fipronil and its metabolites in dead Vespa velutina larvae fed with a mash containing 0.01% fipronil. Quechers-based HPLC-DAD allowed for the determination of the fipronil content in baits. Fipronil and the metabolites fipronil sulfone and fipronil sulfide were identified by GC-MS in extracts of larvae fed with a protein mash containing 0.01% fipronil. The transformation of fipronil into fipronil sulfone inside the larvae and the high toxicity of this metabolite open the possibility to produce protein baits with lower biocide concentrations. | es_ES |
dc.description.sponsorship | Omaira de la Hera thanks the University of the Basque Country (UPV/EHU) for her predoctoral grant. Authors thank the Department of Education of Basque Country Government (projects IT1673-22, PUE 2018_1_0007 and PUE 2021_1_008) and the University of the Basque Country (UPV/EHU) (project US21/35) for financial support. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/es/ | |
dc.subject | QuEChERS | es_ES |
dc.subject | chromatography | es_ES |
dc.subject | fipronil | es_ES |
dc.subject | protein bait Vespa velutina larva | es_ES |
dc.subject | metabolism | es_ES |
dc.title | QuEChERS-Based Method for the Determination of Fipronil in Protein Baits and Vespa velutina Larvae by HPLC-DAD and GC-MS | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2024-11-26T17:43:20Z | |
dc.rights.holder | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2297-8739/11/11/317 | es_ES |
dc.identifier.doi | 10.3390/separations11110317 | |
dc.departamentoes | Química analítica | |
dc.departamentoeu | Kimika analitikoa |
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Except where otherwise noted, this item's license is described as © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).