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Desarrollo del proceso de producción de una vacuna para el tratamiento de la alergia a gramíneas basada en una proteína recombinante
(2020-10-14)
La alergia al polen (polinosis) es una de las alergias más comunes que afecta hasta a un 20 % de la población europea, siendo los pólenes de gramíneas los principales causantes de polinosis. Para el tratamiento de la ...
Quantification of Biomarker Functionality Predicts Patient Outcomes
(Springer Nature, 2021-05-11)
Implementation of a quantitative molecular imaging method (iFRET), which determines receptor–ligand interactions, has led to the finding that patients with a low extent of PD-1/PD-L1 interaction in metastatic NSCLC, and ...
Immunogenomics of Colorectal Cancer Response to Checkpoint Blockade: Analysis of the KEYNOTE 177 Trial and Validation Cohorts
(Elsevier, 2021-10)
BACKGROUND & AIMS: Colorectal cancer (CRC) shows variable response to immune checkpoint blockade, which can only partially be explained by high tumor mutational burden (TMB). We conducted an integrated study of the cancer ...
In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies
(MDPI, 2021-07-24)
Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via ...
Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA
(American Association for the Advancement of Science, 2021-10)
[EN]Glycolipids are prominent components of bacterial membranes that play critical roles not only in maintaining the structural integrity of the cell but also in modulating host-pathogen interactions. PatA is an essential ...
FORTIS: a Live-Cell Assay to Monitor AMPA Receptors Using pH-Sensitive Fluorescence Tags
(Nature, 2021-05-27)
The real-time live fluorescent monitoring of surface AMPA receptors (AMPARs) could open new opportunities for drug
discovery and phenotypic screening concerning neuropsychiatric disorders. We have developed FORTIS, a tool ...
Fluorine Labeling of Nanoparticles and In Vivo 19F Magnetic Resonance Imaging
(American Chemical Society, 2021-03-24)
Fluorinated nanoparticles have increasing applications, but they are still challenging to prepare, especially in the case of water-soluble fluorinated nanoparticles. Herein, a fluorine labeling strategy is presented that ...
MERLIN: a novel BRET-based proximity biosensor for studying mitochondria–ER contact sites
(Life Science Alliance, 2020-01)
The contacts between the ER and mitochondria play a key role in cellular functions such as the exchange of lipids and calcium between both organelles, as well as in apoptosis and autophagy signaling. The molecular architecture ...
Four Cholesterol-Recognition Motifs in the Pore-Forming and Translocation Domains of Adenylate Cyclase Toxin Are Essential for Invasion of Eukaryotic Cells and Lysis of Erythrocytes
(MDPI, 2022)
Adenylate Cyclase Toxin (ACT or CyaA) is one of the important virulence factors secreted by Bordetella pertussis, the bacterium causative of whooping cough. ACT debilitates host defenses by production of unregulated levels ...
Conformational Rearrangements Regulating the DNA Repair Protein APE1
(MDPI, 2022)
Apurinic apyrimidinic endonuclease 1 (APE1) is a key enzyme of the Base Excision Repair (BER) pathway, which primarily manages oxidative lesions of DNA. Once the damaged base is removed, APE1 recognises the resulting abasic ...