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Detection of Atomic Scale Changes in the Free Volume Void Size of Three-Dimensional Colorectal Cancer Cell Culture Using Positron Annihilation Lifetime Spectroscopy

dc.contributor.authorAxpe Iza, Eneko
dc.contributor.authorLópez Euba, Tamara
dc.contributor.authorCastellanos Rubio, Ainara
dc.contributor.authorMérida Sanz, David
dc.contributor.authorGarcía Martínez, José Angel
dc.contributor.authorPlaza-Izurieta, Leticia
dc.contributor.authorFernández-Jiménez, Nora
dc.contributor.authorPlazaola Muguruza, Fernando ORCID
dc.contributor.authorBilbao Catalá, José Ramón ORCID
dc.date.accessioned2014-01-09T19:03:05Z
dc.date.available2014-01-09T19:03:05Z
dc.date.issued2014-01-02
dc.identifier.citationPLoS ONE 9(1) : (2014) // e83838es
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10810/11188
dc.description5 p.es
dc.description.abstractPositron annihilation lifetime spectroscopy (PALS) provides a direct measurement of the free volume void sizes in polymers and biological systems. This free volume is critical in explaining and understanding physical and mechanical properties of polymers. Moreover, PALS has been recently proposed as a potential tool in detecting cancer at early stages, probing the differences in the subnanometer scale free volume voids between cancerous/healthy skin samples of the same patient. Despite several investigations on free volume in complex cancerous tissues, no positron annihilation studies of living cancer cell cultures have been reported. We demonstrate that PALS can be applied to the study in human living 3D cell cultures. The technique is also capable to detect atomic scale changes in the size of the free volume voids due to the biological responses to TGF-β. PALS may be developed to characterize the effect of different culture conditions in the free volume voids of cells grown in vitro.es
dc.description.sponsorshipBasque Department of Industry grant no. SAI11/263 – PRODETEC and Basque Department of Education, Universities and Research grant no. IT/443/10. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectabsorption spectroscopyes
dc.subjectadenocarcinomases
dc.subjectcell cultureses
dc.subjectcell differentiationes
dc.subjectcollagenses
dc.subjectepithelial cellses
dc.subjectimmunofluorescencees
dc.subjectpolymerses
dc.titleDetection of Atomic Scale Changes in the Free Volume Void Size of Three-Dimensional Colorectal Cancer Cell Culture Using Positron Annihilation Lifetime Spectroscopyes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2014 Axpe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0083838es
dc.identifier.doi10.1371/journal.pone.0083838
dc.departamentoesPediatríaes_ES
dc.departamentoesFísica aplicada IIes_ES
dc.departamentoesGenética, antropología física y fisiología animales_ES
dc.departamentoesElectricidad y electrónicaes_ES
dc.departamentoeuPediatriaes_ES
dc.departamentoeuFisika aplikatua IIes_ES
dc.departamentoeuGenetika,antropologia fisikoa eta animalien fisiologiaes_ES
dc.departamentoeuElektrizitatea eta elektronikaes_ES
dc.subject.categoriaAGRICULTURAL AND BIOLOGICAL SCIENCES
dc.subject.categoriaMEDICINE
dc.subject.categoriaBIOCHEMISTRY AND MOLECULAR BIOLOGY


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