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α2,3-Sialyltransferase ST3Gal III Modulates Pancreatic Cancer Cell Motility and Adhesion In Vitro and Enhances Its Metastatic Potential In Vivo

dc.contributor.authorPérez-Garay, Marta
dc.contributor.authorArteta Ruiz, Beatriz ORCID
dc.contributor.authorPagès, Lluís
dc.contributor.authorDe Llorens, Rafael
dc.contributor.authorDe Bolòs, Carme
dc.contributor.authorVidal Vanaclocha, Fernando
dc.contributor.authorPeracaula, Rosa
dc.date.accessioned2011-06-08T14:15:58Z
dc.date.available2011-06-08T14:15:58Z
dc.date.issued2010-09-01
dc.identifier.citationPLoS ONE 5(9) : (2010) // e12524es
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10810/2773
dc.description11 p.es
dc.description.abstractBackground: Cell surface sialylation is emerging as an important feature of cancer cell metastasis. Sialyltransferase expression has been reported to be altered in tumours and may account for the formation of sialylated tumour antigens. We have focused on the influence of alpha-2,3-sialyltransferase ST3Gal III in key steps of the pancreatic tumorigenic process. Methodology/Principal Findings: ST3Gal III overexpressing pancreatic adenocarcinoma cell lines Capan-1 and MDAPanc-28 were generated. They showed an increase of the tumour associated antigen sialyl-Lewis(x). The transfectants' E-selectin binding capacity was proportional to cell surface sialyl-Lewis(x) levels. Cellular migration positively correlated with ST3Gal III and sialyl-Lewis(x) levels. Moreover, intrasplenic injection of the ST3Gal III transfected cells into athymic nude mice showed a decrease in survival and higher metastasis formation when compared to the mock cells. Conclusion: In summary, the overexpression of ST3Gal III in these pancreatic adenocarcinoma cell lines underlines the role of this enzyme and its product in key steps of tumour progression such as adhesion, migration and metastasis formation.es
dc.description.sponsorshipThis work was supported by La Marato de TV3 foundation (grant 050932, awarded to R.P.), Spanish Ministerio de Ciencia e Innovacion (grant BIO 2007-61323, awarded to R.P.), the Government of Catalonia (grant 2005SGR00065, awarded to R.L.), the Comision Interministerial de Ciencia y Tecnologí­a (CICYT) of the Spanish Government in Madrid (no. SAF2006-09341, awarded to F.V.V.) and the Basque Country Government (no. IT-487-07 awarded to F.V.V.). M.P.-G. thanks Fundació La Marató for a pre-doctoral fellowship and University of Girona for a short-term mobility fellowship. The funders have no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.es
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relationinfo:eu-repo/grantAgreement/MICINN/BIO 2007-61323
dc.relationinfo:eu-repo/grantAgreement/MCYT/SAF2006-09341
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectsialyl lewis xes
dc.subjectbeta-galactoside alpha-2,6-sialyltransferasees
dc.subjectsinusoidal endothelial cellses
dc.subjectmessenger rna expressiones
dc.subjecthuman colorectal canceres
dc.subjectnatural killer cellses
dc.subjecthuman hepatocarcinomaes
dc.subjectfucosyl transferasees
dc.subjectbreast canceres
dc.subjecttumor cellses
dc.titleα2,3-Sialyltransferase ST3Gal III Modulates Pancreatic Cancer Cell Motility and Adhesion In Vitro and Enhances Its Metastatic Potential In Vivoes
dc.title.alternativealpha 2,3-Sialyltransferase ST3Gal III Modulates Pancreatic Cancer Cell Motility and Adhesion In Vitro and Enhances Its Metastatic Potential In Vivoes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2010 Pérez-Garay et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0012524es
dc.identifier.doi10.1371/journal.pone.0012524
dc.departamentoesBiología celular e histologíaes_ES
dc.departamentoeuZelulen biologia eta histologiaes_ES
dc.subject.categoriaAGRICULTURAL AND BIOLOGICAL SCIENCES
dc.subject.categoriaMEDICINE
dc.subject.categoriaBIOCHEMISTRY AND MOLECULAR BIOLOGY


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