The Tyrosine Phosphatase hPTPRβ Controls the Early Signals and Dopaminergic Cells Viability via the P2X7 Receptor
dc.contributor.author | Llavero Bernal, Francisco | |
dc.contributor.author | Luque Montoro, Miriam | |
dc.contributor.author | Arrazola Sastre, Alazne | |
dc.contributor.author | Lacerda, Hadriano M. | |
dc.contributor.author | Zugaza Gurruchaga, José Luis | |
dc.date.accessioned | 2021-12-10T11:20:49Z | |
dc.date.available | 2021-12-10T11:20:49Z | |
dc.date.issued | 2021-11-29 | |
dc.identifier.citation | International Journal of Molecular Sciences 22(23) : (2021) // Article ID 12936 | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/10810/54413 | |
dc.description.abstract | ATP, one of the signaling molecules most commonly secreted in the nervous system and capable of stimulating multiple pathways, binds to the ionotropic purinergic receptors, in particular, the P2X7 receptor (P2X7R) and stimulates neuronal cell death. Given this effect of purinergic receptors on the viability of dopaminergic neurons model cells and that Ras GTPases control Erk1/2-regulated mitogen-activated cell proliferation and survival, we have investigated the role of the small GTPases of the Ras superfamily, together with their regulatory and effector molecules as the potential molecular intermediates in the P2X7R-regulated cell death of SN4741 dopaminergic neurons model cells. Here, we demonstrate that the neuronal response to purinergic stimulation involves the Calmodulin/RasGRF1 activation of the small GTPase Ras and Erk1/2. We also demonstrate that tyrosine phosphatase PTPRβ and other tyrosine phosphatases regulate the small GTPase activation pathway and neuronal viability. Our work expands the knowledge on the intracellular responses of dopaminergic cells by identifying new participating molecules and signaling pathways. In this sense, the study of the molecular circuitry of these neurons is key to understanding the functional effects of ATP, as well as considering the importance of these cells in Parkinson’s Disease. | es_ES |
dc.description.sponsorship | This work were supported by: A.A.S. is a recipient of a predoctoral Basque Government fellowship: PRE_2017_1_0016; M.L.M. is a recipient of a fellowship from Foundation “Jesús de Gangoiti y Barrera”: Foundation “Jesús de Gangoiti y Barrera”; J.L.Z. was supported by the Instituto de Salud Carlos III: (PI18/00207); J.L.Z. was supported by the Basque Government: (PIBA_2020_1_0048); J.L.Z. was supported by the University of Basque Country Grant: (US19/04). A.A.S. is a recipient of a predoctoral fellowship (PRE_2017_1_0016) from the Basque Government; M.L.M. is a recipient of a fellowship from Foundation “Jesús de Gangoiti y Barrera”; J.L.Z. was supported by the Instituto de Salud Carlos III (PI18/00207), Basque Government (PIBA_2020_1_0048) and University of Basque Country Grant (US19/04). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | P2X7 receptor | es_ES |
dc.subject | Ras GTPase | es_ES |
dc.subject | Erk1/2 | es_ES |
dc.subject | hPTPRβ | es_ES |
dc.subject | dopaminergic neurons | es_ES |
dc.subject | Parkinson’s | es_ES |
dc.title | The Tyrosine Phosphatase hPTPRβ Controls the Early Signals and Dopaminergic Cells Viability via the P2X7 Receptor | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-12-09T14:32:09Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/22/23/12936/htm | es_ES |
dc.identifier.doi | 10.3390/ijms222312936 | |
dc.departamentoes | Genética, antropología física y fisiología animal | |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia |
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Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).