Local renin angiotensin system and sperm DNA fragmentation.
dc.contributor.author | Aparicio Prieto, Mª Victoria | |
dc.contributor.author | Rodríguez Gallego, María Victoria | |
dc.contributor.author | Valdivia Palacin, Asier | |
dc.contributor.author | Franco Iriarte, Yosu | |
dc.contributor.author | Hervás Bárbara, Gotzone | |
dc.contributor.author | Echevarría Orella, Enrique | |
dc.contributor.author | Casis Sáenz, Luis | |
dc.date.accessioned | 2022-05-17T09:04:15Z | |
dc.date.available | 2022-05-17T09:04:15Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Asian Journal of Andrology 24 : 139-146 (2022) | es_ES |
dc.identifier.issn | 1745-7262 | |
dc.identifier.uri | http://hdl.handle.net/10810/56574 | |
dc.description.abstract | [EN] The renin angiotensin system (RAS) appears to influence male fertility at multiple levels. In this work, we analyzed the relationship between the RAS and DNA integrity. Fifty male volunteers were divided into two groups (25 each): control (DNA fragmentation ≤20%) and pathological (DNA fragmentation >20%) cases. Activities of five peptidases controlling RAS were measured fluorometrically: prolyl endopeptidase (which converts angiotensin [A] I and A II to A 1-7), neutral endopeptidase (NEP/CD10: A I to A 1-7), aminopeptidase N (APN/CD13: A III to A IV), aminopeptidase A (A II to A III) and aminopeptidase B (A III to A IV). Angiotensin-converting enzyme (A I to A II), APN/CD13 and NEP/CD10 were also assessed by semiquantitative cytometry and quantitative flow cytometry assays, as were the receptors of all RAS components: A II receptor type 1 (AT1R), A II receptor type 2 (AT2R), A IV receptor (AT4R or insulin-regulated aminopeptidase [IRAP]), (pro)renin receptor (PRR) and A 1-7 receptor or Mas receptor (MasR) None of the enzymes that regulate levels of RAS components, except for APN/CD13 (decrease in fragmented cells), showed significant differences between both groups. Micrographs of RAS receptors revealed no significant differences in immunolabeling patterns between normozoospermic and fragmented cells. Labeling of AT1R (94.3% normozoospermic vs 84.1% fragmented), AT4R (96.2% vs 95.3%) and MasR (97.4% vs 87.2%) was similar between the groups. AT2R (87.4% normozoospermic vs 63.1% fragmented) and PRR (96.4% vs 48.2%) were higher in non-fragmented spermatozoa. These findings suggest that fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides. | es_ES |
dc.description.sponsorship | This work was supported by grants from the University of the Basque Country (UPV/EHU GIU 17/19) and the Gangoiti Barrera Foundation (Basque Country). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wolters Kluwer | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/es/ | * |
dc.subject | DNA fragmentation | es_ES |
dc.subject | local renin angiotensin system | es_ES |
dc.subject | sperm fertility | es_ES |
dc.title | Local renin angiotensin system and sperm DNA fragmentation. | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | ©The Author(s)(2021). This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. | es_ES |
dc.rights.holder | Atribución-NoComercial-CompartirIgual 3.0 España | * |
dc.relation.publisherversion | https://www.ajandrology.com/article.asp?issn=1008-682X;year=2022;volume=24;issue=2;spage=139;epage=146;aulast=Aparicio | es_ES |
dc.identifier.doi | 10.4103/aja202150 | |
dc.departamentoes | Biología celular e histología | es_ES |
dc.departamentoes | Fisiología | es_ES |
dc.departamentoeu | Fisiologia | es_ES |
dc.departamentoeu | Zelulen biologia eta histologia | es_ES |
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allows others to remix, tweak, and build upon the work non-commercially, as long
as appropriate credit is given and the new creations are licensed under the identical
terms.