Conditioned Medium from H2O2-Preconditioned Human Adipose-Derived Stem Cells Ameliorates UVB-Induced Damage to Human Dermal Fibroblasts
dc.contributor.author | Burón Aizpiri, María | |
dc.contributor.author | Palomares Casado, Teodoro | |
dc.contributor.author | Garrido Pascual, Patricia | |
dc.contributor.author | Herrero de la Parte, Borja | |
dc.contributor.author | García Alonso, Ignacio | |
dc.contributor.author | Alonso Varona, Ana Isabel | |
dc.date.accessioned | 2022-11-04T15:21:10Z | |
dc.date.available | 2022-11-04T15:21:10Z | |
dc.date.issued | 2022-10-11 | |
dc.identifier.citation | Antioxidants 11(10) : (2022) // Article ID 2011 | es_ES |
dc.identifier.issn | 2076-3921 | |
dc.identifier.uri | http://hdl.handle.net/10810/58255 | |
dc.description.abstract | Human skin exposure to ultraviolet B (UVB) radiation can result in acute photodamage through oxidative modifications of cellular components and biomolecules involved in the metabolism of dermal cells. Recently, the therapeutic potential of human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for photoprotection due to their pro-angiogenic properties, protective activity against oxidative stress and paracrine effect on dermal cells. To enhance these therapeutic properties, hASCs can be preconditioned by exposing them to sublethal cellular stressors. In this study, we first analyzed response capacity against UVB-induced oxidative stress in H2O2-preconditioned hASCs (called HC016 cells); and second, we evaluated the photoprotective effect of HC016-conditioned medium (CM) in an in vitro UVB irradiation model in cultured human foreskin fibroblasts (hFFs). The results demonstrated that HC016 cells have a greater capacity to respond efficiently to UVB-induced oxidative stress, evidenced by higher Nrf2 antioxidant system activity and enhanced viability and migration capacity. Further, HC016-CM treatment increased viability, migratory capacity and collagen type I synthesis in hFFs exposed to UVB radiation, as well as reducing their cytotoxicity, apoptosis, senescence and IL-6 secretion. Collectively, these findings support the view that HC016 cells could protect against UVB-induced photodamage via paracrine mechanisms. | es_ES |
dc.description.sponsorship | This research was founded by the University of the Basque Country (UPV/EHU, grant reference numbers: GIU19/088 and PES 21/50). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | photodamage | es_ES |
dc.subject | oxidative stress | es_ES |
dc.subject | human adipose-derived stem cells | es_ES |
dc.subject | dermal fibroblasts | es_ES |
dc.subject | H2O2-preconditioning | es_ES |
dc.subject | ultraviolet B radiation | es_ES |
dc.subject | cell therapy | es_ES |
dc.title | Conditioned Medium from H2O2-Preconditioned Human Adipose-Derived Stem Cells Ameliorates UVB-Induced Damage to Human Dermal Fibroblasts | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2022-10-26T11:08:06Z | |
dc.rights.holder | © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2076-3921/11/10/2011 | es_ES |
dc.identifier.doi | 10.3390/antiox11102011 | |
dc.departamentoes | Cirugía, radiología y medicina física | |
dc.departamentoes | Biología celular e histología | |
dc.departamentoeu | Kirurgia,erradiologia eta medikuntza fisikoa | |
dc.departamentoeu | Zelulen biologia eta histologia |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).