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dc.contributor.authorAlonso Rojas, Rosa María ORCID
dc.contributor.authorBerrueta Simal, Luis Angel
dc.contributor.authorAbad García, Beatriz
dc.contributor.authorSasía-Arriba, Andrea
dc.contributor.authorAsensio Regalado, Carlos ORCID
dc.contributor.authorDapena, Enrique
dc.contributor.authorGallo Hermosa, Blanca ORCID
dc.date.accessioned2023-12-21T15:31:27Z
dc.date.available2023-12-21T15:31:27Z
dc.date.issued2022-06-10
dc.identifier.citationJournal of Agricultural and Food Chemistry 70(41) : 13071-13081(2022)es_ES
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.urihttp://hdl.handle.net/10810/63482
dc.description.abstractLiquid chromatography high-resolution mass spectrometry fingerprinting together with pattern recognition techniques was used to determine the metabolites involved in the susceptibility of apple cultivars to rosy apple aphid (RAA). Preprocessing of ultra-high-performance liquid chromatography coupled to lectrospray ionization and quadrupole time-of-flight mass spectrometry raw data of resistant and susceptible apple cultivars was carried out with XCMS and CAMERA packages. Univariate statistical tools and multivariate data analysis highlighted significant different profiles of the apple metabolomes according to their tolerance to RAA. Optimized and cross-validated Partial least squares discriminant analysis and orthogonal projections to latent structures discriminant analysis models confirmed trans-4-caffeoylquinic acid and 4-p-coumaroylquinic acid as biomarkers for the identification of resistant and susceptible apple cultivars to RAA and disclosed that only hydroxycinnamic acids are involved in the disease susceptibility of cultivars. In this sense, the final steps of the biosynthesis of caffeoylquinic acid (CQA) and p-coumaroylquinic acid (p-CoQA) become decisive because the isomerization of 5-CQA to 4-CQA is favored in resistant cultivars, whereas the isomerization of 5-p-CoQA to 4-p-CoQA is favored in susceptible cultivars.es_ES
dc.description.sponsorshipThis work was supported by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) with European Regional Development Fund (ERDF) funds (Projects RTA2012-00118-C03-01 and -03, RTA2014-00090-C03-01 and -03, and RTA2017-00102-C03-01 and -03).es_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectMalus domesticaes_ES
dc.subjectDysaphis plantagineaes_ES
dc.subjectplant resistancees_ES
dc.subjectUHPLC−QToF/MSes_ES
dc.subjectchemometricses_ES
dc.titleUntargeted Metabolomic Liquid Chromatography High-Resolution Mass Spectrometry Fingerprinting of Apple Cultivars for the Identification of Biomarkers Related to Resistance to Rosy Apple Aphides_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holder© 2022 American Chemical Societyes_ES
dc.relation.publisherversionhttps://doi.org/10.1021/acs.jafc.2c00738es_ES
dc.identifier.doi10.1021/acs.jafc.2c00738
dc.departamentoesQuímica analíticaes_ES
dc.departamentoeuKimika analitikoaes_ES


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