dc.contributor.author | Alonso Rojas, Rosa María | |
dc.contributor.author | Berrueta Simal, Luis Angel | |
dc.contributor.author | Abad García, Beatriz | |
dc.contributor.author | Sasía-Arriba, Andrea | |
dc.contributor.author | Asensio Regalado, Carlos | |
dc.contributor.author | Dapena, Enrique | |
dc.contributor.author | Gallo Hermosa, Blanca | |
dc.date.accessioned | 2023-12-21T15:31:27Z | |
dc.date.available | 2023-12-21T15:31:27Z | |
dc.date.issued | 2022-06-10 | |
dc.identifier.citation | Journal of Agricultural and Food Chemistry 70(41) : 13071-13081(2022) | es_ES |
dc.identifier.issn | 0021-8561 | |
dc.identifier.issn | 1520-5118 | |
dc.identifier.uri | http://hdl.handle.net/10810/63482 | |
dc.description.abstract | Liquid chromatography high-resolution mass spectrometry fingerprinting together with pattern recognition techniques was used to determine the metabolites involved in the susceptibility of apple cultivars to rosy apple aphid (RAA). Preprocessing of ultra-high-performance liquid chromatography coupled to lectrospray ionization and quadrupole time-of-flight mass spectrometry raw data of resistant and susceptible apple cultivars was carried out with XCMS and CAMERA packages. Univariate statistical tools and multivariate data analysis highlighted significant different profiles of the apple metabolomes according to their tolerance to RAA. Optimized and cross-validated Partial least squares discriminant analysis and orthogonal projections to latent structures discriminant analysis models confirmed trans-4-caffeoylquinic acid and 4-p-coumaroylquinic acid as biomarkers for the identification of resistant and susceptible apple cultivars to RAA and disclosed that only hydroxycinnamic acids are involved in the disease susceptibility of cultivars. In this sense, the final steps of the biosynthesis of caffeoylquinic acid (CQA) and p-coumaroylquinic acid (p-CoQA) become decisive because the isomerization of 5-CQA to 4-CQA is favored in resistant cultivars, whereas the isomerization of 5-p-CoQA to 4-p-CoQA is favored in susceptible cultivars. | es_ES |
dc.description.sponsorship | This work was supported by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) with European Regional Development Fund (ERDF) funds (Projects RTA2012-00118-C03-01 and -03, RTA2014-00090-C03-01 and -03, and RTA2017-00102-C03-01 and -03). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Malus domestica | es_ES |
dc.subject | Dysaphis plantaginea | es_ES |
dc.subject | plant resistance | es_ES |
dc.subject | UHPLC−QToF/MS | es_ES |
dc.subject | chemometrics | es_ES |
dc.title | Untargeted Metabolomic Liquid Chromatography High-Resolution Mass Spectrometry Fingerprinting of Apple Cultivars for the Identification of Biomarkers Related to Resistance to Rosy Apple Aphid | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 American Chemical Society | es_ES |
dc.relation.publisherversion | https://doi.org/10.1021/acs.jafc.2c00738 | es_ES |
dc.identifier.doi | 10.1021/acs.jafc.2c00738 | |
dc.departamentoes | Química analítica | es_ES |
dc.departamentoeu | Kimika analitikoa | es_ES |