Gene delivery in the cornea: in vitro and ex vivo evaluation of solid lipid nanoparticle-based vectors
Fecha
2018-08-01Autor
Vicente Pascual, Mónica
Albano, Andrea
Serpe, Loredana
Rodríguez Gascón, Alicia
Foglietta, Loredana
Muntoni, Elisabetta
Torrecilla, Josune
Battaglia, Luigi
Metadatos
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Nanomedicine 13(15) : 1847-1854 (2018)
Resumen
Inflammation is a process that underlies sight-threatening ocular surface
diseases, and gene supplementation with the plasmid that encodes for interleukin-10 (p-
IL10) will allow the sustained de novo synthesis of the cytokine to occur in corneal
cells, and provide a long-term anti-inflammatory effect. This work describes the
development of solid lipid nanoparticle (SLN) systems for the delivery of p-IL10 to
transfect the cornea. Results: In vitro, vectors showed suitable features as non-viral
vectors (size, zeta potential, DNA binding, protection and release), and they were able
to enter and transfect human corneal epithelial cells. Ex vivo, the vectors were found to
transfect the epithelium, the stroma and the endothelium in rabbit corneal explants.
Distribution of gene expression within the cell layers of the cornea depended on the
composition of the four vectors evaluated. Conclusion: SLN-based vectors are
promising gene delivery systems for corneal diseases, including inflammation.