dc.contributor.author | Da Silva Ferrada, Elisa | |
dc.contributor.author | Xolalpa, Wendy | |
dc.contributor.author | Lang, Valerie | |
dc.contributor.author | Aillet, Fabienne | |
dc.contributor.author | Martín-Ruiz, Itziar | |
dc.contributor.author | De la Cruz-Herrera, Carlos F. | |
dc.contributor.author | Lopitz Otsoa, Fernando | |
dc.contributor.author | Carracedo Pérez, Arkaitz | |
dc.contributor.author | Goldenberg, Seth J. | |
dc.contributor.author | Rivas, Carmen | |
dc.contributor.author | England, Patrick | |
dc.contributor.author | Rodríguez, Manuel S. | |
dc.date.accessioned | 2014-02-08T09:28:42Z | |
dc.date.available | 2014-02-08T09:28:42Z | |
dc.date.issued | 2013-04-22 | |
dc.identifier.citation | Scientific Reports 3 : (2013) // Article N. 1690 | es |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10810/11390 | |
dc.description | 6 p. [+ 7 p. Supplementary Information] | es |
dc.description.abstract | SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps to capture endogenous SUMOylated proteins. Our results show that these small peptides are transferable motifs that maintain their SUMO binding capacity when fused to the heterologous carrier protein GST. The tandem disposition of SIMs increases the binding capacity of SUMO-traps to specifically interact with polySUMO but not poly-Ubiquitin chains. We demonstrate that this SUMO capturing system purifies SUMOylated proteins such as I kappa B alpha, PTEN, PML or p53 in vitro and in vivo. These properties can be used to explore the many critical functions regulated by protein SUMOylation | es |
dc.description.sponsorship | We would like to thank C. Gwizdek, M. Matunis and R.T. Hay for reagents and tools provided. This work was funded by the MINECO (Spain) grant BFU2008-01108/BMC and BFU2011-28536 (MSR), Department of Industry of the Government of the Basque Country (Etortek Research Programmes 2011/2012). Our group at Inbiomed is supported by the "Obra Social KUTXA'' and the Diputacion Foral de Guipuzcoa. E.DS-F is fellow from Fundacao para a Ciencia e Tecnologia, Ministerio da Educacao e Ciencia-Portugal, Grant SFRH/BD/71514/2010. | es |
dc.language.iso | eng | es |
dc.publisher | Nature Publishing Group | es |
dc.relation | info:eu-repo/grantAgreement/MINECO/BFU2008-01108 | |
dc.relation | info:eu-repo/grantAgreement/MINECO/BFU2011-28536 | |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | protein enrichment | es |
dc.subject | sumoylation | es |
dc.subject | oncogenes | es |
dc.subject | stress signalling | es |
dc.subject | in-vivo | es |
dc.subject | motif | es |
dc.subject | identification | es |
dc.subject | degradation | es |
dc.subject | proteases | es |
dc.subject | RNF4 | es |
dc.subject | PML | es |
dc.subject | P53 | es |
dc.title | Analysis of SUMOylated proteins using SUMO-traps | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | This work is licensed under a Creative Commons
Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this
license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ | es |
dc.relation.publisherversion | http://www.nature.com/srep/2013/130422/srep01690/full/srep01690.html | es |
dc.identifier.doi | 10.1038/srep01690 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
dc.subject.categoria | MULTIDISCIPLINARY SCIENCES | |