Now showing items 1-20 of 241

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      A Computational Module Assembled from Different Protease Family Motifs Identifies PI PLC from Bacillus cereus as a Putative Prolyl Peptidase with a Serine Protease Scaffold 

      Rendón Ramírez, Adela; Shukla, Manish; Oda, Masataka; Chakraborty, Sandeep; Minda, Renu; Dandekar, Abhaya M.; Asgeirsson, Bjarni; Goñi Urcelay, Félix María ORCID; Rao, Basuthkar J. (Public Library Science, 2013-08)
      Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types have been systematically categorized in the MEROPS database. While a BLAST search on these proteases identifies homologous ...
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      A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis 

      Barth, Nicole D.; Subirós Funosas, Ramón; Mendive Tapia, Lorena; Duffin, Rodger; Shields, Mario A.; Cartwright, Jennifer A.; Troeira Henriques, Sónia; Sot, Jesús; Goñi Urcelay, Félix María ORCID; Lavilla, Rodolfo; Marwick, John A.; Vermeren, Sonja; Rossi, Adriano G.; Egeblad, Mikala; Dransfield, Ian; Vendrell, Marc (Nature, 2020-08-12)
      Programmed cell death or apoptosis is a central biological process that is dysregulated in many diseases, including inflammatory conditions and cancer. The detection and quantification of apoptotic cells in vivo is hampered ...
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      A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines 

      Peris, Leticia; Bisbal, Mariano; Martínez Hernández, José ORCID; Saoudi, Yasmina; Jonckheere, Julie; Rolland, Marta; Sebastien, Muriel; Brocard, Jacques; Denarier, Eric; Bosc, Christophe; Guerin, Christophe; Gory-Fauré, Sylvie; Deloulme, Jean Christophe; Lanté, Fabien; Arnal, Isabelle; Buisson, Alain; Goldberg, Yves; Blanchoin, Laurent (Nature Publishing, 2018-09-17)
      Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects ...
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      A long non-coding RNA that harbors a SNP associated with type 2 diabetes regulates the expression of TGM2 gene in pancreatic beta cells 

      González Moro, Itziar ORCID; Rojas Márquez, Henar; Sebastián de la Cruz, Maialen ORCID; Mentxaka Salgado, Jon; Olazagoitia Garmendia, Ane; Mendoza Gómez, Luis Manuel; Lluch, Aina; Fantuzzi, Federica; Lambert, Carmen; Ares Blanco, Jessica; Marselli, Lorella; Marchetti, Piero; Cnop, Miriam; Delgado, Elías; Fernández Real, José Manuel; Ortega, Francisco José; Castellanos Rubio, Ainara; Santín Gómez, Izortze ORCID (Frontiers Media, 2023-02)
      IntroductionMost of the disease-associated single nucleotide polymorphisms (SNPs) lie in non- coding regions of the human genome. Many of these variants have been predicted to impact the expression and function of long ...
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      A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation 

      Castellanos Rubio, Ainara; Santín Gómez, Izortze ORCID; Olazagoitia Garmendia, Ane; Romero Garmendia, Irati; Jauregi Miguel, Amaia ORCID; Legarda, Maria; Bilbao Catalá, José Ramón ORCID (Nature Publishing Group, 2019-12)
      N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches ...
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      A Proteomic Approach for Systematic Mapping of Substrates of Human Deubiquitinating Enzymes 

      Ramírez Sánchez, Juan Manuel ORCID; Prieto Agujeta, Gorka ORCID; Olazabal Herrero, Ane; Borràs, Eva; Fernandez-Vigo, Elvira; Alduntzin Egizurain, Unai ORCID; Osinalde Moraleja, Nerea ORCID; Beaskoetxea Lejarzegi, Javier; Lectez, Benoît ORCID; Aloria Escolastico, Kerman; Rodríguez Pérez, José Antonio ORCID; Paradela, Alberto; Sabidó, Eduard; Muñoz, Javier; Corrales, Fernando; Arizmendi Bastarrika, Jesús María ORCID; Mayor Martínez, Ugo ORCID (MDPI, 2021-05-03)
      The human genome contains nearly 100 deubiquitinating enzymes (DUBs) responsible for removing ubiquitin moieties from a large variety of substrates. Which DUBs are responsible for targeting which substrates remain mostly ...
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      A Systematic Approach to Assess the Activity and Classification of PCSK9 Variants 

      Belloso Uribe, Kepa; Chemello, Kevin; Larrea Sebal, Asier; Benito Vicente, Asier ORCID; Bourane, Steeve; Jaafar, Ali K.; Lambert, Gilles; Martín Plágaro, César Augusto ORCID (MDPI, 2021-12-18)
      Background: Gain of function (GOF) mutations of PCSK9 cause autosomal dominant familial hypercholesterolemia as they reduce the abundance of LDL receptor (LDLR) more efficiently than wild-type PCSK9. In contrast, PCSK9 ...
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      Aberrant Synaptic PTEN in Symptomatic Alzheimer's Patients May Link Synaptic Depression to Network Failure 

      Díaz González, Marta; Buberman, Assaf; Morales Fuciños, Miguel; Ferrer, Isidro; Knafo Farhi, Dina Shira (Frontiers Media, 2021-05-11)
      In Alzheimer's disease (AD), Amyloid beta (A beta) impairs synaptic function by inhibiting long-term potentiation (LTP), and by facilitating long-term depression (LTD). There is now evidence from AD models that A beta ...
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      Actin- regulated Siglec- 1 nanoclustering influences HIV-1 capture and virus- containing compartment formation in dendritic cells 

      Gutiérrez Martínez, Enric; Benet Garrabé, Susana; Mateos, Nicolás; Erkizia, Itziar; Nieto Garai, Jon Ander ORCID; Lorizate Nogales, Maier ORCID; Borgman, Kyra J.E.; Manzo, Carlo; Campelo, Félix; Izquierdo Useros, Nuria; Martínez Picado, Javier; García Parajo, María F. (eLife Sciences, 2023-03)
      The immunoglobulin-like lectin receptor CD169 (Siglec-1) mediates the capture of HIV- 1 by activated dendritic cells (DCs) through binding to sialylated ligands. These interactions result in a more efficient virus capture ...
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      Activation of the DnaK-ClpB Complex is Regulated by the Properties of the Bound Substrate 

      Fernández Higuero, José Ángel ORCID; Aguado Martínez, Alejandra; Perales Calvo, Judit; Moro Pérez, Fernando ORCID; Muga Villate, Arturo ORCID (Nature Publishing, 2018-04-11)
      The chaperone ClpB in bacteria is responsible for the reactivation of aggregated proteins in collaboration with the DnaK system. Association of these chaperones at the aggregate surface stimulates ATP hydrolysis, which ...
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      Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells 

      Martín Plágaro, César Augusto ORCID; Etxaniz Iriondo, Asier ORCID; Belloso Uribe, Kepa; Etxebarria Gallego, Aitor; González Bullón, David ORCID; Arluzea de Jauregizar, Jon Andoni ORCID; Goñi Urcelay, Félix María ORCID; Arechaga Martínez, Juan Miguel ORCID; Ostolaza Echabe, Elena Amaya ORCID (Nature Publishing Group, 2015-09-08)
      Bordetella pertussis causes whooping cough, a respiratory infectious disease that is the fifth largest cause of vaccine-preventable death in infants. Though historically considered an extracellular pathogen, this bacterium ...
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      Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity 

      Martín Plágaro, César Augusto ORCID; Belloso Uribe, Kepa; Gómez Bilbao, Geraxane; Ostolaza Echabe, Elena Amaya ORCID (Public Library of Science, 2011-02-23)
      Bordetella pertussis, the bacterium that causes whooping cough, secretes an adenylate cyclase toxin (ACT) that must be post-translationally palmitoylated in the bacterium cytosol to be active. The toxin targets phagocytes ...
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      Advantages and Versatility of Fluorescence-Based Methodology to Characterize the Functionality of LDLR and Class Mutation Assignment 

      Echevarria Gallego, Aitor; Benito Vicente, Asier ORCID; Ostolaza Echabe, Elena Amaya ORCID; Martín Plágaro, César Augusto ORCID (Public Library Science, 2014-11-11)
      Familial hypercholesterolemia (FH) is a common autosomal codominant disease with a frequency of 1:500 individuals in its heterozygous form. The genetic basis of FH is most commonly mutations within the LDLR gene. Assessing ...
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      Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments 

      Rujas Díez, Edurne; Insausti González, Sara; Leaman, Daniel P.; Carravilla Palomanes, Pablo ORCID; González Resines, Saul; Monceaux, Valérie; Sánchez Eugenia, Rubén ORCID; García Porras, Miguel; Iloro, Ibon; Zhang, Lei; Elortza, Felix; Julien, Jean Philippe; Sáez Cirión, Asier; Zwick, Michael B.; Eggeling, Christian; Ojida, Akio; Domene, Carmen; Caaveiro, Jose M.M.; Nieva Escandón, José Luis ORCID (Cell Press, 2020-08-18)
      The contribution of membrane interfacial interactions to recognition of membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization of this type of antibodies via chemical ...
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      Age, Origin and Functional Study of the Prevalent LDLR Mutation Causing Familial Hypercholesterolaemia in Gran Canaria 

      Suárez, Nicolás M.; Jebari Benslaiman, Shifa; Jiménez-Monzón, Roberto; Benito Vicente, Asier ORCID; Brito-Casillas, Yeray; Garcés, Laida; González-Lleo, Ana M.; Tugores, Antonio; Boronat, Mauro; Martín Plágaro, César Augusto ORCID; Wägner, Ana M.; Sánchez-Hernández, Rosa M. (MDPI, 2023-07-11)
      The p.(Tyr400_Phe402del) mutation in the LDL receptor (LDLR) gene is the most frequent cause of familial hypercholesterolaemia (FH) in Gran Canaria. The aim of this study was to determine the age and origin of this prevalent ...
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      Agricultural Soils Amended with Thermally-Dried Anaerobically-Digested Sewage Sludge Showed Increased Risk of Antibiotic Resistance Dissemination 

      Jauregi, Leire; Epelde Sierra, Lur; Alcorta Calvo, Miren Itziar; Garbisu Crespo, Carlos (Frontiers Media, 2021-04-28)
      The application of sewage sludge (SS) to agricultural soil can help meet crop nutrient requirements and enhance soil properties, while reusing an organic by-product. However, SS can be a source of antibiotic resistance ...
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      An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles 

      Madsen, Thomas D.; Hansen, Lasse H.; Hintze, John; Ye, Zilu; Jebari Benslaiman, Shifa; Andersen, Daniel B.; Joshi, Hiren J.; Ju, Tongzhong; Goetze, Jens P.; Martin, Cesar; Rosenkilde, Mette M.; Holst, Jens J.; Kuhre, Rune E.; Goth, Christoffer K.; Vakhrushev, Sergey Y.; Schjoldager, Katrine T. (Nature, 2020-08-20)
      Peptide hormones and neuropeptides encompass a large class of bioactive peptides that regulate physiological processes like anxiety, blood glucose, appetite, inflammation and blood pressure. Here, we execute a focused ...
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      An E2F7-Dependent Transcriptional Program Modulates DNA Damage Repair And Genomic Stability 

      Michelena Sánchez, Jone ORCID; Apraiz García, Aintzane ORCID; Vallejo Rodríguez, Jon ORCID; García Santisteban, Iraia ORCID; Fullaondo Elordui-Zapaterieche, Asier ORCID; Alvarez Fernández, Mónica; Marcos, Marcos; Zubiaga Elordieta, Ana María ORCID (Oxford University Press, 2018-05-18)
      The cellular response to DNA damage is essential for maintaining the integrity of the genome. Recent evidence has identified E2F7 as a key player in DNA damage-dependent transcriptional regulation of cell-cycle genes. ...
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      An Epilepsy-Causing Mutation Leads to Co-Translational Misfolding of the Kv7.2 Channel 

      Urrutia Iñiguez, Janire ORCID; Aguado Martínez, Alejandra; Gomis Pérez, Carolina; Muguruza Montero, Arantza; Rodríguez Ballesteros, Oscar; Zhang, Jiaren; Núñez Viadero, Eider ORCID; Malo de la Fuente, Covadonga; Chung, Hee Jung; Leonardo Liceranzu, Aritz ORCID; Bergara Jauregui, Aitor; Villarroel Muñoz, Álvaro (Springer, 2021-05-21)
      BACKGROUND: The amino acid sequence of proteins generally carries all the necessary information for acquisition of native conformations, but the vectorial nature of translation can additionally determine the folding outcome. ...
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      Analysis of SUMOylated proteins using SUMO-traps 

      Da Silva Ferrada, Elisa; Xolalpa, Wendy; Lang, Valerie; Aillet, Fabienne; Martín-Ruiz, Itziar; De la Cruz-Herrera, Carlos F.; Lopitz Otsoa, Fernando; Carracedo Pérez, Arkaitz ORCID; Goldenberg, Seth J.; Rivas, Carmen; England, Patrick; Rodríguez, Manuel S. (Nature Publishing Group, 2013-04-22)
      SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps to capture endogenous SUMOylated proteins. Our results show ...