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dc.contributor.authorRodríguez Gascón, Alicia
dc.contributor.authorDel Pozo Rodríguez, Ana ORCID
dc.contributor.authorSolinís Aspiazu, María Ángeles ORCID
dc.date.accessioned2016-04-04T14:53:33Z
dc.date.available2016-04-04T14:53:33Z
dc.date.issued2014
dc.identifier.citationInternational Journal of Nanomedicine 9 : 1833-1843(2014)es
dc.identifier.issn1178-2013
dc.identifier.urihttp://hdl.handle.net/10810/17776
dc.description.abstractSelf-amplifying RNA or RNA replicon is a form of nucleic acid-based vaccine derived from either positive-strand or negative-strand RNA viruses. The gene sequences encoding structural proteins in these RNA viruses are replaced by mRNA encoding antigens of interest as well as by RNA polymerase for replication and transcription. This kind of vaccine has been successfully assayed with many different antigens as vaccines candidates, and has been shown to be potent in several animal species, including mice, nonhuman primates, and humans. A key challenge to realizing the broad potential of self-amplifying vaccines is the need for safe and effective delivery methods. Ideally, an RNA nanocarrier should provide protection from blood nucleases and extended blood circulation, which ultimately would increase the possibility of reaching the target tissue. The delivery system must then be internalized by the target cell and, upon receptor-mediated endocytosis, must be able to escape from the endosomal compartment into the cell cytoplasm, where the RNA machinery is located, while avoiding degradation by lysosomal enzymes. Further, delivery systems for systemic administration ought to be well tolerated upon administration. They should be safe, enabling the multiadministration treatment modalities required for improved clinical outcomes and, from a developmental point of view, production of large batches with reproducible specifications is also desirable. In this review, the concept of self-amplifying RNA vaccines and the most promising lipid-based delivery systems are discussed.es
dc.description.sponsorshipThis work was supported by the Basque Government's Department of Education, Universities and Investigation (IT-341-10).es
dc.language.isoenges
dc.publisherDove Medical Presses
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectself-amplifying RNA vaccinees
dc.subjectRNA replicones
dc.subjectliposomeses
dc.subjectsolid lipid nanoparticleses
dc.subjectnucleic acid vaccineses
dc.subjectdirect gene-transferes
dc.subjectmessenger RNAes
dc.subjectin-vivoes
dc.subjectmetastatic melanomaes
dc.subjectalphavirus replicones
dc.subjectnonviral vectorses
dc.subjectsirna deliveryes
dc.subjectvaccination triales
dc.subjectcell transfectiones
dc.subjectdirect-injectiones
dc.titleDevelopment of nucleic acid vaccines: use of self-amplifying RNA in lipid nanoparticleses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2014 Rodríguez-Gascón et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.phpes
dc.relation.publisherversionhttps://www.dovepress.com/development-of-nucleic-acid-vaccines-use-of-self-amplifying-rna-in-lip-peer-reviewed-article-IJN#es
dc.identifier.doi10.2147/IJN.S39810
dc.departamentoesFarmacia y ciencias de los alimentoses_ES
dc.departamentoeuFarmazia eta elikagaien zientziakes_ES
dc.subject.categoriaBIOENGINEERING
dc.subject.categoriaMATERIALS SCIENCE, BIOMATERIALS
dc.subject.categoriaDRUG DISCOVERY
dc.subject.categoriaCHEMISTRY, ORGANIC
dc.subject.categoriaBIOPHYSICS


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