dc.contributor.author | Albaina Vivanco, Aitor | |
dc.contributor.author | Aguirre Rodrigo, Mikel | |
dc.contributor.author | Abad García, David | |
dc.contributor.author | Santos, María | |
dc.contributor.author | Estomba Recalde, Miren Andone | |
dc.date.accessioned | 2016-05-12T13:10:05Z | |
dc.date.available | 2016-05-12T13:10:05Z | |
dc.date.issued | 2016-03 | |
dc.identifier.citation | Ecology and Evolution 6(6) : 1809-1824 (2016) | es |
dc.identifier.issn | 2045-7758 | |
dc.identifier.uri | http://hdl.handle.net/10810/18240 | |
dc.description.abstract | The potential of the 18S rRNA V9 metabarcoding approach for diet assessment was explored using MiSeq paired-end (PE; 2 9 150 bp) technology. To critically evaluate the method's performance with degraded/digested DNA, the diets of two zooplanktivorous fish species from the Bay of Biscay, European sardine (Sardina pilchardus) and European sprat (Sprattus sprattus), were analysed. The taxonomic resolution and quantitative potential of the 18S V9 metabarcoding was first assessed both in silico and with mock and field plankton samples. Our method was capable of discriminating species within the reference database in a reliable way providing there was at least one variable position in the 18S V9 region. Furthermore, it successfully discriminated diet between both fish species, including habitat and diel differences among sardines, overcoming some of the limitations of traditional visual-based diet analysis methods. The high sensitivity and semi-quantitative nature of the 18S V9 metabarcoding approach was supported by both visual microscopy and qPCR-based results. This molecular approach provides an alternative cost and time effective tool for food-web analysis. | es |
dc.description.sponsorship | The sampling was financially supported by the projects ECOGENBAY (MICINN CTM2009-13570-C02-02), funded by the Government of Spain, and the project "Bay of Biscay anchovy biomass estimates for year 2010", funded by the Basque Government and by the European Commission within the frame of the National Sampling Programme (DCF No. 665/2008 14 July 2008). The General Secretariat of Sea also collaborated providing the R/V Emma Bardan. AA and DA work was supported by a contract with the Euskampus Foundation (Euskampus Fundazioa) and a doctoral fellowship of the Basque Government (UPV/EHU "ZabaldUz" program), respectively. This study was funded by a Basque Government grant to "Grupo de Investigacion consolidado del sistema universitario vasco, IT558-10". | es |
dc.language.iso | eng | es |
dc.publisher | Wiley Blackwell | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | 18S rRNA V9 | es |
dc.subject | diet analysis | es |
dc.subject | metabarcoding | es |
dc.subject | MiSeq paired-end technology | es |
dc.subject | plankton | es |
dc.subject | sardina pilchardus | es |
dc.subject | sprattus sprattus | es |
dc.subject | real-time PCR | es |
dc.subject | high throughput | es |
dc.subject | copy munber | es |
dc.subject | north-sea | es |
dc.subject | DNA | es |
dc.subject | biodiversity | es |
dc.subject | communities | es |
dc.subject | diversity | es |
dc.subject | copepods | es |
dc.title | 18S rRNA V9 metabarcoding for diet characterization: a critical evaluation with two sympatric zooplanktivorous fish species | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | 2016 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use,
distribution and reproduction in any medium, provided the original work is properly cited. | es |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/ece3.1986/abstract?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+14th+May+11:00-14:00+BST+/+06:00-09:00+EDT+/+18:00-21:00+SGT+for+essential+maintenance.Apologies+for+the+inconvenience. | es |
dc.identifier.doi | 10.1002/ece3.1986 | |
dc.departamentoes | Genética, antropología física y fisiología animal | es_ES |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | es_ES |