dc.contributor.author | Hervás Hidalgo, Javier ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Landajuela Larma, Ane | |
dc.contributor.author | Antón Helas, Zuriñe | |
dc.contributor.author | Shnyrova Zhadan, Anna ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Goñi Urcelay, Félix María ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Alonso Izquierdo, Alicia ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.date.accessioned | 2018-04-18T08:50:07Z | |
dc.date.available | 2018-04-18T08:50:07Z | |
dc.date.issued | 2017-11-15 | |
dc.identifier.citation | Scientific Reports 7 : (2017) // Article ID 15614 | es_ES |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10810/26407 | |
dc.description.abstract | Specific protein-lipid interactions lead to a gradual recruitment of AuTophaGy-related (ATG) proteins to the nascent membrane during autophagosome (AP) formation. ATG3, a key protein in the movement of LC3 towards the isolation membrane, has been proposed to facilitate LC3/GABARAP lipidation in highly curved membranes. In this work we have performed a biophysical study of human ATG3 interaction with membranes containing phosphatidylethanolamine, phosphatidylcholine and anionic phospholipids. We have found that ATG3 interacts more strongly with negatively-charged phospholipid vesicles or nanotubes than with electrically neutral model membranes, cone-shaped anionic phospholipids (cardiolipin and phosphatidic acid) being particularly active in promoting binding. Moreover, an increase in membrane curvature facilitates ATG3 recruitment to membranes although addition of anionic lipid molecules makes the curvature factor relatively less important. The predicted N-terminus amphipathic a-helix of ATG3 would be responsible for membrane curvature detection, the positive residues Lys 9 and 11 being essential in the recognition of phospholipid negative moieties. We have also observed membrane aggregation induced by ATG3 in vitro, which could point to a more complex function of this protein in AP biogenesis. Moreover, in vitro GABARAP lipidation assays suggest that ATG3-membrane interaction could facilitate the lipidation of ATG8 homologues. | es_ES |
dc.description.sponsorship | This article is part of COST (European Cooperation in Science and Technology) Actions (PROTEOSTASIS, BM1307, TRANSAUTOPHAGY, CA15138). The authors thank Dr. Isei Tanida (National Institute of Infectious Diseases, Tokyo, Japan) for providing human ATG3 and GABARAP plasmids, and to Dr. Martin B. Ulmschneider (Johns Hopkins University, Baltimore, MD) for Fig. 1B. They are also indebted to Ms Araceli Marcos for technical support. This work was supported in part by grants from the Spanish Ministry of Economy and FEDER (BFU 2011-28566, BFU 2015-66306-P, AGL2011-24758), and from the Basque Government (IT838-13, IT84913). A.S. acknowledges support from RyC Program of the Spanish Ministry of Economy. J.H.H and Z.A. were predoctoral students supported by the University of the Basque Country.
Editorial | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/ | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | excess membrane reservoir | es_ES |
dc.subject | autophagosome formation | es_ES |
dc.subject | mitochondrial homeostasis | es_ES |
dc.subject | atg12-atg5 conjugate | es_ES |
dc.subject | mammalian autophagy | es_ES |
dc.subject | supported bilayers | es_ES |
dc.subject | lc3 conjugation | es_ES |
dc.subject | curvature | es_ES |
dc.subject | proteins | es_ES |
dc.subject | cholesterol | es_ES |
dc.title | Human ATG3 binding to lipid bilayers: role of lipid geometry, and electric charge | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-017-15057-6 | es_ES |
dc.identifier.doi | 10.1038/s41598-017-15057-6 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |