Are miRNA-103, miRNA-107 and miRNA-122 Involved in the Prevention of Liver Steatosis Induced by Resveratrol?
dc.contributor.author | Gracia Jadraque, Ana | |
dc.contributor.author | Fernández Quintela, Alfredo | |
dc.contributor.author | Miranda Gómez, Jonatan | |
dc.contributor.author | Eseberri Barace, Itziar | |
dc.contributor.author | González, Marcela | |
dc.contributor.author | Portillo Baquedano, María Puy | |
dc.date.accessioned | 2018-06-12T12:32:49Z | |
dc.date.available | 2018-06-12T12:32:49Z | |
dc.date.issued | 2017-04 | |
dc.identifier.citation | Nutrients 9(4) : (2017) // Article ID 360 | es_ES |
dc.identifier.issn | 2072-6643 | |
dc.identifier.uri | http://hdl.handle.net/10810/27494 | |
dc.description.abstract | The aim of the present study was to determine whether the reduction in liver fat previously observed in our laboratory in a cohort of rats which had been fed an obesogenic diet was mediated by changes in the expression of microRNA (miRNA)-103-3p, miRNA-107-3p and miRNA-122-5p, which represent 70% of total miRNAs in the liver, as well as in their target genes. The expression of the three analysed miRNAs was reduced in rats treated with resveratrol. A reduction in sterol-regulatory element binding protein 1 (SREBP1) and an increase in carnitine palmitoyltransferase 1a (CPT1a) were observed in resveratrol-treated rats. No changes were found in fatty acid synthase (FAS). In cultured hepatocytes, SREBP1 protein was increased after the transfection of each miRNA. FAS protein expression was decreased after the transfection of miRNA-122-5p, and CPT1a protein was down-regulated by the over-expression of miRNA-107-3p. This study provides new evidences which show that srebf1 is a target gene for miRNA-103-3p and miRNA-107-3p, fasn a target gene for miRNA-122-5p and cpt1a a target gene for miRNA-107-3p. Moreover, the reduction in liver steatosis induced by resveratrol in rats fed an obesegenic diet is mediated, at least in part, by the increase in CPT1a protein expression and activity, via a decrease in miRNA-107-3p expression. | es_ES |
dc.description.sponsorship | This study was supported by grants from the Ministerio de Economia y Competitividad (AGL-2015-65719-FEDER-UE), Instituto de Salud Carlos III (CIBERobn), Government of the Basque Country (IT-572-13) and University of the Basque Country (UPV/EHU) (ELDUNANOTEK UFI11/32). A. Gracia is a PhD fellowship from the Ministerio de Economia y Competitividad. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/AGL-2015-65719-FEDER-UE | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | miRNA-103 | es_ES |
dc.subject | miRNA-107 | es_ES |
dc.subject | miRNA-122 | es_ES |
dc.subject | steatosis | es_ES |
dc.subject | liver | es_ES |
dc.subject | resveratrol | es_ES |
dc.subject | rat | es_ES |
dc.subject | white adipose-tissue | es_ES |
dc.subject | metabolic syndrome | es_ES |
dc.subject | hepatic steatosis | es_ES |
dc.subject | lipid-metabolism | es_ES |
dc.subject | obesogenic diet | es_ES |
dc.subject | gene-expression | es_ES |
dc.subject | fat | es_ES |
dc.subject | supplementation | es_ES |
dc.title | Are miRNA-103, miRNA-107 and miRNA-122 Involved in the Prevention of Liver Steatosis Induced by Resveratrol? | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | http://www.mdpi.com/2072-6643/9/4/360 | es_ES |
dc.identifier.doi | 10.3390/nu9040360 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | es_ES |
dc.departamentoeu | Farmazia eta elikagaien zientziak | es_ES |
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