Understanding the Mechanism of Translocation of Adenylate Cyclase Toxin across Biological Membranes
dc.contributor.author | Ostolaza Echabe, Elena Amaya | |
dc.contributor.author | Martín Plágaro, César Augusto | |
dc.contributor.author | González Bullón, David ![]() | |
dc.contributor.author | Belloso Uribe, Kepa | |
dc.contributor.author | Etxaniz Iriondo, Asier | |
dc.date.accessioned | 2019-01-08T14:52:39Z | |
dc.date.available | 2019-01-08T14:52:39Z | |
dc.date.issued | 2017-09-21 | |
dc.identifier.citation | Toxins 9(10) : (2017) // Article ID 295 | es_ES |
dc.identifier.issn | 2072-6651 | |
dc.identifier.uri | http://hdl.handle.net/10810/30665 | |
dc.description.abstract | Adenylate cyclase toxin (ACT) is one of the principal virulence factors secreted by the whooping cough causative bacterium Bordetella pertussis, and it has a critical role in colonization of the respiratory tract and establishment of the disease. ACT targets phagocytes via binding to the CD11b/CD18 integrin and delivers its N-terminal adenylate cyclase (AC) domain directly to the cell cytosol, where it catalyzes unregulated conversion of cytosolic ATP into cAMP upon activation by binding to cellular calmodulin. High cAMP levels disrupt bactericidal functions of the immune cells, ultimately leading to cell death. In spite of its relevance in the ACT biology, the mechanism by which its ≈400 amino acid-long AC domain is transported through the target plasma membrane, and is released into the target cytosol, remains enigmatic. This article is devoted to refresh our knowledge on the mechanism of AC translocation across biological membranes. Two models, the so-called “two-step model” and the recently-proposed “toroidal pore model”, will be considered. | es_ES |
dc.description.sponsorship | This study was supported by grants from the Basque Government (Grupos Consolidados IT849-13 and ETORTEK Program KK-2015/0000089). A.E. was recipient of a fellowship from the University of the Basque Country (UPV/EH) and D.G.-B. was recipient of a fellowship from the Bizkaia Biophysics Foundation. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | adenylate cyclase | es_ES |
dc.subject | RTX toxin | es_ES |
dc.subject | protein translocation | es_ES |
dc.subject | phospholipase activity | es_ES |
dc.subject | model membranes | es_ES |
dc.title | Understanding the Mechanism of Translocation of Adenylate Cyclase Toxin across Biological Membranes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.mdpi.com/2072-6651/9/10/295 | es_ES |
dc.identifier.doi | 10.3390/toxins9100295 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
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Except where otherwise noted, this item's license is described as © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).