A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
dc.contributor.author | Peris, Leticia | |
dc.contributor.author | Bisbal, Mariano | |
dc.contributor.author | Martínez Hernández, José ![]() | |
dc.contributor.author | Saoudi, Yasmina | |
dc.contributor.author | Jonckheere, Julie | |
dc.contributor.author | Rolland, Marta | |
dc.contributor.author | Sebastien, Muriel | |
dc.contributor.author | Brocard, Jacques | |
dc.contributor.author | Denarier, Eric | |
dc.contributor.author | Bosc, Christophe | |
dc.contributor.author | Guerin, Christophe | |
dc.contributor.author | Gory-Fauré, Sylvie | |
dc.contributor.author | Deloulme, Jean Christophe | |
dc.contributor.author | Lanté, Fabien | |
dc.contributor.author | Arnal, Isabelle | |
dc.contributor.author | Buisson, Alain | |
dc.contributor.author | Goldberg, Yves | |
dc.contributor.author | Blanchoin, Laurent | |
dc.date.accessioned | 2019-01-11T12:57:14Z | |
dc.date.available | 2019-01-11T12:57:14Z | |
dc.date.issued | 2018-09-17 | |
dc.identifier.citation | Nature Communications 9 : (2018) // Article ID 3775 | es_ES |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | http://hdl.handle.net/10810/30758 | |
dc.description.abstract | Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses. | es_ES |
dc.description.sponsorship | We are grateful to zootechnicians of the Grenoble Institute of Neurosciences, S. Andrieu, M. Lapierre, F. Mehr and F. Rimet for animal care. We thank N. Collomb, C. Corrao, F. Vossier and C. Paoli for technical assistance; T. Rush and M. Seggio for introducing L. P. to FRAP, TIRF microscopy; Ju Brocard and G. Falivelli for helping with virus production and Fondation Bettencourt Schueller for equipment. This work was supported by INSERM, CEA, Universite Grenoble Alpes and in part by awards from the French Agence Nationale de la Recherche to A. A. (2010- Blanc- 120201 CBioS); to A.B., A. A., I. A. (2011-MALZ-001-0217 MALAAD) and to A. A., I. A. (2017-CE11-0026 MAMAs). This work was supported by the Photonic Imaging Center of Grenoble Institute Neuroscience (Univ Grenoble Alpes - Inserm U1216) which is part of the ISdV core facility and certified by the IBiSA label. PhD stipends were from the French Ministry for Research and Higher Education for M. R., from AFM-Telethon for M. S. and from Region Auvergne Rhones Alpes for J.J. M.B. was a recipient of the Roche Pharmaceutic RPF program (AA team and F Hoffmann-La Roche Ltd, Basel, Switzerland). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | long-term potentiation | es_ES |
dc.subject | bundles f-actin | es_ES |
dc.subject | synaptic plasticity | es_ES |
dc.subject | dynamic microtubule | es_ES |
dc.subject | binding; tau | es_ES |
dc.subject | growth | es_ES |
dc.subject | stop | es_ES |
dc.subject | phosphorylation | es_ES |
dc.subject | depolymerization | es_ES |
dc.title | A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s41467-018-05869-z | es_ES |
dc.identifier.doi | 10.1038/s41467-018-05869-z | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
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