dc.contributor.author | Alberdi Alfonso, Elena María | |
dc.contributor.author | Weldon, John E. | |
dc.contributor.author | Becerra, S. Patricia | |
dc.date.accessioned | 2019-03-22T18:12:52Z | |
dc.date.available | 2019-03-22T18:12:52Z | |
dc.date.issued | 2003-02-19 | |
dc.identifier.citation | BMC Biochemistry 4: (2003) // Article ID 1 | es_ES |
dc.identifier.issn | 1471-2091 | |
dc.identifier.uri | http://hdl.handle.net/10810/32120 | |
dc.description.abstract | BACKGROUND: Pigment epithelium-derived factor (PEDF) has binding affinity for cell-surface receptors in retinoblastoma cells and for glycosaminoglycans. We investigated the effects of glycosaminoglycans on PEDF-receptor interactions.
RESULTS: 125I-PEDF formed complexes with protease-resistant components of medium conditioned by human retinoblastoma Y-79 cells. Using specific glycosaminoglycan degrading enzymes in spectrophotometric assays and PEDF-affinity chromatography, we detected heparin and heparan sulfate-like glycosaminoglycans in the Y-79 conditioned media, which had binding affinity for PEDF. The Y-79 conditioned media significantly enhanced the binding of 125I-PEDF to Y-79 cell-surface receptors. However, enzymatic and chemical depletion of sulfated glycosaminoglycans from the Y-79 cell cultures by heparitinase and chlorate treatments decreased the degree of 125I-PEDF binding to cell-surface receptors.
CONCLUSIONS: These data indicate that retinoblastoma cells secrete heparin/heparan sulfate with binding affinity for PEDF, which may be important in efficient cell-surface receptor binding. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.title | Glycosaminoglycans in human retinoblastoma cells: heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2003 Alberdi et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. | es_ES |
dc.relation.publisherversion | https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-4-1 | es_ES |
dc.identifier.doi | 10.1186/1471-2091-4-1 | |
dc.departamentoes | Neurociencias | es_ES |
dc.departamentoeu | Neurozientziak | es_ES |