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dc.contributor.authorTrobajo Sanmartín, Camino ORCID
dc.contributor.authorEzpeleta Lobato, Guillermo
dc.contributor.authorPais, Célia
dc.contributor.authorEraso Barrio, María Elena ORCID
dc.contributor.authorQuindós Andrés, Guillermo
dc.date.accessioned2019-04-01T13:14:17Z
dc.date.available2019-04-01T13:14:17Z
dc.date.issued2018-09-29
dc.identifier.citationBMC Genomics 19 : (2018) // Article ID 718es_ES
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/10810/32296
dc.description.abstractBackground: Analysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR. To achieve this, the results obtained using the new protocol were compared to the ones obtained using a previously described reference method. To that end, diagnostic accuracy, informativeness and discrimination parameters were estimated. Results: Our results showed good concordance between both methods (Kappa index: 0.920), leading to a high sensitivity (1; CI(95%) (0.991-1)) and specificity (1; CI(95%) (0.772-1)) after the validation of the new protocol. Moreover, the electropherograms profiles obtained with the new PCR scheme showed a high signal to noise ratio (SNR). Conclusions: The new multiplex protocol is valuable for the differentiation of C. parapsilosis sensu stricto, with direct clinical applications. Besides, the new protocol represents a shortening the hands-on time, reducing the sample manipulation (dismissing the possibility of cross-contamination), maintaining the quality of the results (when compared to the ones obtained with the reference method), and helping to the standardization and simplification of the genotyping scheme.es_ES
dc.description.sponsorshipThis work was supported by the Consejeria de Educacion, Universidades e Investigacion of the Gobierno Vasco-Eusko Jaurlaritza [GIC 15/78 IT-990-16] and the Universidad del Pais Vasco/Euskal Herriko Unibertsitatea [UFI 11/25]. CTS has been supported by a research grant of the Fundacion Jesus de Gangoiti Barrera.es_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectcandida parapsilosis sensu strictoes_ES
dc.subjectgenotyping techniquees_ES
dc.subjectmicrosatellites repeatses_ES
dc.subjectmultiplex pcres_ES
dc.subjectspecificityes_ES
dc.subjectreproducibility of resultses_ES
dc.subjectintensive-care-unites_ES
dc.subjectamplificationes_ES
dc.subjectdiversityes_ES
dc.subjectstrainses_ES
dc.subjectmarkerses_ES
dc.titleDesign and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolateses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-5065-3es_ES
dc.identifier.doi10.1186/s12864-018-5065-3
dc.departamentoesInmunología, microbiología y parasitologíaes_ES
dc.departamentoesMedicina preventiva y salud públicaes_ES
dc.departamentoeuImmunologia, mikrobiologia eta parasitologiaes_ES
dc.departamentoeuPrebentzio medikuntza eta osasun publikoaes_ES


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.