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Histone deacetylase inhibitors induce invasion of human melanoma cells in vitro via differential regulation of N-cadherin expression and RhoA activity
dc.contributor.author | Díaz Núñez, María | |
dc.contributor.author | Díez Torre, Alejandro | |
dc.contributor.author | De Wever, Olivier | |
dc.contributor.author | Andrade Pocino, Ricardo | |
dc.contributor.author | Arluzea de Jauregizar, Jon Andoni | |
dc.contributor.author | Silió López, Margarita Rosa | |
dc.contributor.author | Arechaga Martínez, Juan Miguel | |
dc.date.accessioned | 2019-04-10T10:39:49Z | |
dc.date.available | 2019-04-10T10:39:49Z | |
dc.date.issued | 2016-08-22 | |
dc.identifier.citation | BMC Cancer 16 : (2016) // Article ID 667 | es_ES |
dc.identifier.issn | 1471-2407 | |
dc.identifier.uri | http://hdl.handle.net/10810/32406 | |
dc.description.abstract | Background: Histone deacetylase inhibitors (HDACi) exert multiple cytotoxic actions on cancer cells. Currently, different synthetic HDACi are in clinical use or clinical trials; nevertheless, since both pro-invasive and anti-invasive activities have been described, there is some controversy about the effect of HDACi on melanoma cells. Methods: Matrigel and Collagen invasion assays were performed to evaluate the effect of several HDACi (Butyrate, Trichostatin A, Valproic acid and Vorinostat) on two human melanoma cell line invasion (A375 and HT-144). The expression of N- and E-Cadherin and the activity of the RhoA GTPase were analyzed to elucidate the mechanisms involved in the HDACi activity. Results: HDACi showed a pro-invasive effect on melanoma cells in vitro. This effect was accompanied by an up-regulation of N-cadherin expression and an inhibition of RhoA activity. Moreover, the down-regulation of N-cadherin through blocking antibodies or siRNA abrogated the pro-invasive effect of the HDACi and, additionally, the inhibition of the Rho/ROCK pathway led to an increase of melanoma cell invasion similar to that observed with the HDACi treatments. Conclusion: These results suggest a role of N-cadherin and RhoA in HDACi induced invasion and call into question the suitability of some HDACi as antitumor agents for melanoma patients. | es_ES |
dc.description.sponsorship | Research grants from the Spanish Ministry of Economy and Competitiveness (SAF2012-39773), the regional Basque Government Research Groups Program (T560-10) and the SAIOTEK Program of the Basque Science, Technology & Innovation net (SPRI) to J. Arechaga supported this work. M. Diaz-Nunez held a fellowship from the University of the Basque Country and, later on, from the Jesus Gangoiti Barrera Foundation of Bilbao. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Biomed Central | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2012-39773 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | histone deacetylase inhibitors | es_ES |
dc.subject | HDACi | es_ES |
dc.subject | melanoma | es_ES |
dc.subject | cell invasion | es_ES |
dc.subject | N-cadherin | es_ES |
dc.subject | Rhoa | es_ES |
dc.subject | epithelial-mesenchymal transitions | es_ES |
dc.subject | cancer-cells | es_ES |
dc.subject | breast-cancer | es_ES |
dc.subject | carcinoma metastasis | es_ES |
dc.subject | pancreatic-cancer | es_ES |
dc.subject | gene-expression | es_ES |
dc.subject | HDAC inhibitors | es_ES |
dc.subject | valproic | es_ES |
dc.subject | lung-cancer | es_ES |
dc.subject | stem-cells | es_ES |
dc.title | Histone deacetylase inhibitors induce invasion of human melanoma cells in vitro via differential regulation of N-cadherin expression and RhoA activity | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2693-3 | es_ES |
dc.identifier.doi | 10.1186/s12885-016-2693-3 | |
dc.departamentoes | Biología celular e histología | es_ES |
dc.departamentoeu | Zelulen biologia eta histologia | es_ES |
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