Gaining ecological insight on dietary allocation among horseshoe bats through molecular primer combination
dc.contributor.author | Aldasoro Lecea, Miren | |
dc.contributor.author | Garín Atorrasagasti, Ignacio | |
dc.contributor.author | Vallejo López, Nerea | |
dc.contributor.author | Baroja Ibañez de Maeztu, Unai | |
dc.contributor.author | Arrizabalaga Escudero, Aitor | |
dc.contributor.author | Goiti Ugarte, Urtzi | |
dc.contributor.author | Aiartza Azurtza, José Ramón | |
dc.date.accessioned | 2020-02-28T09:50:12Z | |
dc.date.available | 2020-02-28T09:50:12Z | |
dc.date.issued | 2019-07-24 | |
dc.identifier.citation | Plos One 14(7) : (2019) // Article ID e0220081 | es_ES |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10810/41853 | |
dc.description.abstract | Knowledge on the trophic interactions among predators and their prey is important in order to understand ecology and behaviour of animals. Traditionally studies on the diet composition of insectivorous bats have been based on the morphological identification of prey remains, but the accuracy of the results has been hampered due to methodological limitations. Lately, the DNA metabarcoding and High Throughput Sequencing (HTS) techniques have changed the scene since they allows prey identification to the species level, ultimately giving more precision to the results. Nevertheless, the use of one single primer set to amplify faecal DNA produces biases in the assessed dietary composition. Three horseshoe bats overlap extensively in their distribution range in Europe: Rhinolophus euryale, R. hipposideros and R. ferrumequinum. In order to achieve the deepest insight on their prey list we combined two different primers. Results showed that the used primers were complementary at the order and species levels, only 22 out of 135 prey species being amplified by both. The most frequent prey of R. hipposideros belonged to Diptera and Lepidoptera, to Lepidoptera in R. euryale, and Lepidoptera, Diptera and Coleoptera in R. ferrumequinum. The three bats show significant resource partitioning, since their trophic niche overlap is not higher than 34%. Our results confirm the importance of combining complementary primers to describe the diet of generalist insectivorous bats with amplicon metabarcoding techniques. Overall, each primer set showed a subset of the prey composition, with a small portion of the total prey being identified by both of them. Therefore, each primer presented a different picture of the niche overlap among the three horseshoe bats due to their taxonomic affinity. | es_ES |
dc.description.sponsorship | The Research was supported by the Basque Government (project No IT754-13, PI: Joxerra Aihartza) and the Spanish Government (CGL2012-38610, PI: Inazio Garin; and CGL2015-69069-P,PI: Urtzi Goiti). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | desman galemys-pyrenaicus | es_ES |
dc.subject | dna-based identification | es_ES |
dc.subject | rhinolophus-hipposideros | es_ES |
dc.subject | prey selection | es_ES |
dc.subject | chiroptera | es_ES |
dc.subject | pcr | es_ES |
dc.title | Gaining ecological insight on dietary allocation among horseshoe bats through molecular primer combination | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2019 Aldasoro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0220081 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0220081 | |
dc.departamentoes | Zoología y biología celular animal | es_ES |
dc.departamentoeu | Zoologia eta animalia zelulen biologia | es_ES |
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Except where otherwise noted, this item's license is described as © 2019 Aldasoro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.