Wnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cells
dc.contributor.author | Uribe-Echevarria Zubizarreta, Verónica | |
dc.contributor.author | García Gallastegui, Patricia ![]() | |
dc.contributor.author | Pérez Garrastachu, Miguel | |
dc.contributor.author | Casado Andrés, María del Rosario ![]() | |
dc.contributor.author | Irastorza Epelde, Igor ![]() | |
dc.contributor.author | Unda Rodríguez, Fernando José ![]() | |
dc.contributor.author | Ibarretxe Bilbao, Gaskon ![]() | |
dc.contributor.author | Subirán Ciudad, Nerea ![]() | |
dc.date.accessioned | 2020-04-02T18:13:30Z | |
dc.date.available | 2020-04-02T18:13:30Z | |
dc.date.issued | 2020-03-07 | |
dc.identifier.citation | Cells 9(3) : (2020) // Article ID 652 | es_ES |
dc.identifier.issn | 2073-4409 | |
dc.identifier.uri | http://hdl.handle.net/10810/42591 | |
dc.description.abstract | Dental pulp stem cells (DPSCs) from adult teeth show the expression of a very complete repertoire of stem pluripotency core factors and a high plasticity for cell reprogramming. Canonical Wnt and Notch signaling pathways regulate stemness and the expression of pluripotency core factors in DPSCs, and even very short-term (48 h) activations of the Wnt pathway induce a profound remodeling of DPSCs at the physiologic and metabolic levels. In this work, DPSC cultures were exposed to treatments modulating Notch and Wnt signaling, and also induced to differentiate to osteo/adipocytes. DNA methylation, histone acetylation, histone methylation, and core factor expression levels where assessed by mass spectroscopy, Western blot, and qPCR. A short-term activation of Wnt signaling by WNT-3A induced a genomic DNA demethylation, and increased histone acetylation and histone methylation in DPSCs. The efficiency of cell reprogramming methods relies on the ability to surpass the epigenetic barrier, which determines cell lineage specificity. This study brings important information about the regulation of the epigenetic barrier by Wnt signaling in DPSCs, which could contribute to the development of safer and less aggressive reprogramming methodologies with a view to cell therapy. | es_ES |
dc.description.sponsorship | This work was funded by the UPV/EHU (GIU16/66, UFI 11/44; to F.U.), the Basque Government (GV/EJ; Ikerketa Taldeak IT831-13; to G.I. and ELKARTEK KK-2019-00093; to F.U.) and ISCIII (DTS18/00142; to N.S.). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | dental pulp stem cells | es_ES |
dc.subject | chromatin remodeling | es_ES |
dc.subject | cell cycle | es_ES |
dc.subject | pluripotency | es_ES |
dc.subject | DNA methylation | es_ES |
dc.subject | histone acetylation | es_ES |
dc.subject | histone methylation | es_ES |
dc.subject | Notch pathway | es_ES |
dc.subject | Wnt pathway | es_ES |
dc.title | Wnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2020-03-27T14:53:40Z | |
dc.rights.holder | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2073-4409/9/3/652 | es_ES |
dc.identifier.doi | 10.3390/cells9030652 | |
dc.departamentoes | Biología celular e histología | |
dc.departamentoes | Fisiología | |
dc.departamentoeu | Zelulen biologia eta histologia | |
dc.departamentoeu | Fisiologia |
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Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)