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dc.contributor.authorUribe-Echevarria Zubizarreta, Verónica
dc.contributor.authorGarcía Gallastegui, Patricia ORCID
dc.contributor.authorPérez Garrastachu, Miguel
dc.contributor.authorCasado Andrés, María del Rosario ORCID
dc.contributor.authorIrastorza Epelde, Igor ORCID
dc.contributor.authorUnda Rodríguez, Fernando José ORCID
dc.contributor.authorIbarretxe Bilbao, Gaskon ORCID
dc.contributor.authorSubirán Ciudad, Nerea ORCID
dc.date.accessioned2020-04-02T18:13:30Z
dc.date.available2020-04-02T18:13:30Z
dc.date.issued2020-03-07
dc.identifier.citationCells 9(3) : (2020) // Article ID 652es_ES
dc.identifier.issn2073-4409
dc.identifier.urihttp://hdl.handle.net/10810/42591
dc.description.abstractDental pulp stem cells (DPSCs) from adult teeth show the expression of a very complete repertoire of stem pluripotency core factors and a high plasticity for cell reprogramming. Canonical Wnt and Notch signaling pathways regulate stemness and the expression of pluripotency core factors in DPSCs, and even very short-term (48 h) activations of the Wnt pathway induce a profound remodeling of DPSCs at the physiologic and metabolic levels. In this work, DPSC cultures were exposed to treatments modulating Notch and Wnt signaling, and also induced to differentiate to osteo/adipocytes. DNA methylation, histone acetylation, histone methylation, and core factor expression levels where assessed by mass spectroscopy, Western blot, and qPCR. A short-term activation of Wnt signaling by WNT-3A induced a genomic DNA demethylation, and increased histone acetylation and histone methylation in DPSCs. The efficiency of cell reprogramming methods relies on the ability to surpass the epigenetic barrier, which determines cell lineage specificity. This study brings important information about the regulation of the epigenetic barrier by Wnt signaling in DPSCs, which could contribute to the development of safer and less aggressive reprogramming methodologies with a view to cell therapy.es_ES
dc.description.sponsorshipThis work was funded by the UPV/EHU (GIU16/66, UFI 11/44; to F.U.), the Basque Government (GV/EJ; Ikerketa Taldeak IT831-13; to G.I. and ELKARTEK KK-2019-00093; to F.U.) and ISCIII (DTS18/00142; to N.S.).es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/
dc.subjectdental pulp stem cellses_ES
dc.subjectchromatin remodelinges_ES
dc.subjectcell cyclees_ES
dc.subjectpluripotencyes_ES
dc.subjectDNA methylationes_ES
dc.subjecthistone acetylationes_ES
dc.subjecthistone methylationes_ES
dc.subjectNotch pathwayes_ES
dc.subjectWnt pathwayes_ES
dc.titleWnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2020-03-27T14:53:40Z
dc.rights.holder© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)es_ES
dc.relation.publisherversionhttps://www.mdpi.com/2073-4409/9/3/652es_ES
dc.identifier.doi10.3390/cells9030652
dc.departamentoesBiología celular e histología
dc.departamentoesFisiología
dc.departamentoeuZelulen biologia eta histologia
dc.departamentoeuFisiologia


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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)