Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
dc.contributor.author | Xie, Yujie | |
dc.contributor.author | Arno, Maria C. | |
dc.contributor.author | Husband, Jonathan T. | |
dc.contributor.author | Torrent Sucarrat, Miquel | |
dc.contributor.author | O’Reilly, Rachel K. | |
dc.date.accessioned | 2020-06-17T11:50:21Z | |
dc.date.available | 2020-06-17T11:50:21Z | |
dc.date.issued | 2020-05-18 | |
dc.identifier.citation | Nature Communications 11 : (2020) // Article ID 2460 | es_ES |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | http://hdl.handle.net/10810/43990 | |
dc.description.abstract | Fluorescent barcoding is a pivotal technique for the investigation of the microscale world, from information storage to the monitoring of dynamic biochemical processes. Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods. However, the use of fluorescence lifetime in this area has been limited by the lack of strategies that provide spatiotemporal manipulation of the coding process. In this study, we design a two-component photo-switchable nanogel that exhibits variable fluorescence lifetime upon photoisomerization-induced energy transfer processes through light irradiation. This remotely manipulated fluorescence lifetime property could be visually mapped using fluorescence lifetime imaging microscopy (FLIM), allowing selective storage and display of information at the microscale. Most importantly, the reversibility of this system further provides a strategy for minimizing the background influence in fluorescence lifetime imaging of live cells and sub-cellular organelles. Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods. Here, the authors design a photo-switchable nanogel exhibiting variable fluorescence lifetime, and demonstrate visual mapping by using fluorescence lifetime imaging microscopy on a sub-cellular scale. | es_ES |
dc.description.sponsorship | This work was supported by the ERC (grant number 615142), EPSRC, and the University of Birmingham, the Ministerio de Economia y Competitividad (MINECO) of Spain (project CTQ2016-80375-P) and the Basque Government (grant IT-324-07). The authors acknowledge the computational and technical and human support provided by DIPC. Y.X. acknowledges Chancellor's International Scholarship (University ofWarwick) for funding. All three reviewers are thanked for their time and contribution to the final version of this paper. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/CTQ2016-80375-P | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | energy-transfer; nanoparticles; lanthanide; dithiomaleimide; polymers; contrast; family; bright; probes; fret | es_ES |
dc.title | Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s41467-020-16297-3 | es_ES |
dc.identifier.doi | 10.1038/s41467-020-16297-3 | |
dc.departamentoes | Química orgánica I | es_ES |
dc.departamentoeu | Kimika organikoa I | es_ES |
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