Expansion of Human Limbal Epithelial Stem/Progenitor Cells Using Different Human Sera: A Multivariate Statistical Analysis
dc.contributor.author | Hernáez Moya, Raquel | |
dc.contributor.author | González, Sheyla | |
dc.contributor.author | Urkaregi Etxepare, Arantza | |
dc.contributor.author | José Ignacio Pijoan Zubizarreta | |
dc.contributor.author | Deng, Sophie X. | |
dc.contributor.author | Andollo Victoriano, María Noelia | |
dc.date.accessioned | 2020-09-30T07:57:22Z | |
dc.date.available | 2020-09-30T07:57:22Z | |
dc.date.issued | 2020-07-25 | |
dc.identifier.citation | International Journal of Molecular Sciences 21(17) : (2020) // Article ID 6132 | es_ES |
dc.identifier.issn | 1422-0067, | |
dc.identifier.uri | http://hdl.handle.net/10810/46288 | |
dc.description.abstract | Transplantation of human cultured limbal epithelial stem/progenitor cells (LESCs) has demonstrated to restore the integrity and functionality of the corneal surface in about 76% of patients with limbal stem cell deficiency. However, there are different protocols for the expansion of LESCs, and many of them use xenogeneic products, being a risk for the patients’ health. We compared the culture of limbal explants on the denuded amniotic membrane in the culture medium—supplemental hormone epithelial medium (SHEM)—supplemented with FBS or two differently produced human sera. Cell morphology, cell size, cell growth rate, and the expression level of differentiation and putative stem cell markers were examined. Several bioactive molecules were quantified in the human sera. In a novel approach, we performed a multivariate statistical analysis of data to investigate the culture factors, such as differently expressed molecules of human sera that specifically influence the cell phenotype. Our results showed that limbal cells cultured with human sera grew faster and contained similar amounts of small-sized cells, higher expression of the protein p63α, and lower of cytokeratin K12 than FBS cultures, thus, maintaining the stem/progenitor phenotype of LESCs. Furthermore, the multivariate analysis provided much data to better understand the obtaining of different cell phenotypes as a consequence of the use of different culture methodologies or different culture components. | es_ES |
dc.description.sponsorship | Research study supported by grants from Mutua Madrileña Foundation (FMM11/02), Basque Foundation for Health Research and Innovation BIOEF (BIO14/TP/002), National Eye Institute (R01EY021797), California Institute for Regenerative Medicine (TR-TR2-01768 and CLIN1-08686) and an unrestricted grant from Research to Prevent Blindness to the Department of Ophthalmology, University of California, Los Angeles. R.H.-M. was supported by fellowships from the University of the Basque Country UPV/EHU and the Jesus de Gangoiti Barrera foundation. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | limbal epithelial stem/progenitor cells (LESC) | es_ES |
dc.subject | limbal stem cell deficiency (LSCD) | es_ES |
dc.subject | cell therapy | es_ES |
dc.subject | xenogeneic-free cell expansion | es_ES |
dc.subject | human serum (HS) | es_ES |
dc.subject | serum derived from plasma rich in growth factors (s-PRGF) | es_ES |
dc.subject | human amniotic membrane (HAM) | es_ES |
dc.subject | explants culture | es_ES |
dc.subject | multivariate statistical analysis | es_ES |
dc.subject | principal component analysis (PCA) combined with clusters analysis | es_ES |
dc.subject | partial least squares discriminant analysis (PLS-DA) | es_ES |
dc.title | Expansion of Human Limbal Epithelial Stem/Progenitor Cells Using Different Human Sera: A Multivariate Statistical Analysis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2020-09-07T13:46:26Z | |
dc.rights.holder | 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/21/17/6132 | es_ES |
dc.identifier.doi | 10.3390/ijms21176132 | |
dc.departamentoes | Matemática aplicada | |
dc.departamentoes | Biología celular e histología | |
dc.departamentoeu | Matematika aplikatua | |
dc.departamentoeu | Zelulen biologia eta histologia |
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Except where otherwise noted, this item's license is described as 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).