dc.contributor.author | Sendino Mouliet, Maria ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Omaetxebarria Ibarra, Miren Josu | |
dc.contributor.author | Prieto Agujeta, Gorka ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Rodríguez Pérez, José Antonio ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.date.accessioned | 2020-09-30T09:58:25Z | |
dc.date.available | 2020-09-30T09:58:25Z | |
dc.date.issued | 2020-09-01 | |
dc.identifier.citation | International Journal of Molecular Sciences 21(17) : (2020) // Article ID 6341 | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/10810/46291 | |
dc.description.abstract | The nuclear export receptor CRM1 (XPO1) recognizes and binds specific sequence motifs termed nuclear export signals (NESs) in cargo proteins. About 200 NES motifs have been identified, but over a thousand human proteins are potential CRM1 cargos, and most of their NESs remain to be identified. On the other hand, the interaction of NES peptides with the “NES-binding groove” of CRM1 was studied in detail using structural and biochemical analyses, but a better understanding of CRM1 function requires further investigation of how the results from these in vitro studies translate into actual NES export in a cellular context. Here we show that a simple cellular assay, based on a recently described reporter (SRV<sub>B/A</sub>), can be applied to identify novel potential NESs motifs, and to obtain relevant information on different aspects of CRM1-mediated NES export. Using cellular assays, we first map 19 new sequence motifs with nuclear export activity in 14 cancer-related proteins that are potential CRM1 cargos. Next, we investigate the effect of mutations in individual NES-binding groove residues, providing further insight into CRM1-mediated NES export. Finally, we extend the search for CRM1-dependent NESs to a recently uncovered, but potentially vast, set of small proteins called micropeptides. By doing so, we report the first NES-harboring human micropeptides. | es_ES |
dc.description.sponsorship | This work was supported by grants from the Spanish Government MINECO-FEDER (SAF2014-57743-R), the Basque Country Government (IT1257-19) and the University of the Basque Country (UFI11/20), as well as a fellowship from the Basque Country Government (to M.S.). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2014-57743-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | CRM1 | es_ES |
dc.subject | XPO1 | es_ES |
dc.subject | NES | es_ES |
dc.subject | micropeptide | es_ES |
dc.subject | cellular assay | es_ES |
dc.subject | nuclear export | es_ES |
dc.subject | nuclear export signal | es_ES |
dc.title | Using a Simple Cellular Assay to Map NES Motifs in Cancer-Related Proteins, Gain Insight into CRM1-Mediated NES Export, and Search for NES-Harboring Micropeptides | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2020-09-07T13:46:45Z | |
dc.rights.holder | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/21/17/6341 | es_ES |
dc.identifier.doi | 10.3390/ijms21176341 | |
dc.departamentoes | Genética, antropología física y fisiología animal | |
dc.departamentoes | Bioquímica y biología molecular | |
dc.departamentoes | Ingeniería de comunicaciones | |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | |
dc.departamentoeu | Biokimika eta biologia molekularra | |
dc.departamentoeu | Komunikazioen ingeniaritza | |