Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
dc.contributor.author | Mora Boza, Ana | |
dc.contributor.author | López Ruiz, Elena | |
dc.contributor.author | López Donaire, María Luisa | |
dc.contributor.author | Jiménez, Gema | |
dc.contributor.author | Aguilar, María Rosa | |
dc.contributor.author | Marchal, Juan Antonio | |
dc.contributor.author | Pedraz Muñoz, José Luis | |
dc.contributor.author | Vázquez Lasa, Blanca | |
dc.contributor.author | San Román, Julio | |
dc.contributor.author | Gálvez Martín, Patricia | |
dc.date.accessioned | 2020-12-11T11:47:40Z | |
dc.date.available | 2020-12-11T11:47:40Z | |
dc.date.issued | 2020-11-11 | |
dc.identifier.citation | Polymers 12(11) : (2020) // Article ID 2661 | es_ES |
dc.identifier.issn | 2073-4360 | |
dc.identifier.uri | http://hdl.handle.net/10810/48947 | |
dc.description.abstract | In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant differences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an effective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field. | es_ES |
dc.description.sponsorship | The authors thanks to “La Caixa” Foundation (ID 100010434), which supported Ana Mora-Boza (scholarship code LCF/BQ/ES16/11570018) and to the Spanish Ministry of Economy and Competitiveness for financial support (project RTC-2016-5451-1) and the Fundación Mutua Madrileña (project FMM-AP17196-2019). M. R. Aguilar and B. Vázquez-Lasa are members of the SusPlast platform (Interdisciplinary Platform for Sustainable Plastics towards a Circular Economy) from the Spanish National Research Council (CSIC). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/RTC-2016-5451-1 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | interpenetrated polymer network | es_ES |
dc.subject | semi-IPN | es_ES |
dc.subject | methacrylated hyaluronic acid | es_ES |
dc.subject | chitosan | es_ES |
dc.subject | glycerylphytate | es_ES |
dc.subject | mesenchymal stem cell | es_ES |
dc.title | Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2020-11-26T14:10:45Z | |
dc.rights.holder | 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2073-4360/12/11/2661/htm | es_ES |
dc.identifier.doi | 10.3390/polym12112661 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | |
dc.departamentoeu | Farmazia eta elikagaien zientziak |
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Except where otherwise noted, this item's license is described as 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).