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dc.contributor.authorGonzález Ramírez, Emilio José
dc.contributor.authorGarcía Arribas, Aritz ORCID
dc.contributor.authorSot, Jesús
dc.contributor.authorGoñi Urcelay, Félix María ORCID
dc.contributor.authorAlonso Izquierdo, Alicia
dc.date.accessioned2020-12-23T10:49:31Z
dc.date.available2020-12-23T10:49:31Z
dc.date.issued2020-08-24
dc.identifier.citationScientific Reports 10 : (2020) // Article ID 14085es_ES
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10810/49228
dc.description.abstractThe biophysical properties of sphingolipids containing lignoceric (C24:0) or nervonic (C24:1) fatty acyl residues have been studied in multicomponent lipid bilayers containing cholesterol (Chol), by means of confocal microscopy, differential scanning calorimetry and atomic force microscopy. Lipid membranes composed of dioleoyl phosphatidylcholine and cholesterol were prepared, with the addition of different combinations of ceramides (C24:0 and/or C24:1) and sphingomyelins (C24:0 and/or C24:1). Results point to C24:0 sphingolipids, namely lignoceroyl sphingomyelin (lSM) and lignoceroyl ceramide (lCer), having higher membrane rigidifying properties than their C24:1 homologues (nervonoyl SM, nSM, or nervonoyl Cer, nCer), although with a similar strong capacity to induce segregated gel phases. In the case of the lSM-lCer multicomponent system, the segregated phases have a peculiar fibrillar or fern-like morphology. Moreover, the combination of C24:0 and C24:1 sphingolipids generates interesting events, such as a generalized bilayer dynamism/instability of supported planar bilayers. In some cases, these sphingolipids give rise to exothermic curves in thermograms. These peculiar features were not present in previous studies of C24:1 combined with C16:0 sphingolipids. Conclusions of our study point to nSM as a key factor governing the relative distribution of ceramides when both lCer and nCer are present. The data indicate that lCer could be easier to accommodate in multicomponent bilayers than its C16:0 counterpart. These results are relevant for events of membrane platform formation, in the context of sphingolipid-based signaling cascades.es_ES
dc.description.sponsorshipEGR and AGA were postdoctoral scientists supported by the University of the Basque Country (UPV/EHU). This work was supported in part by the Spanish Ministerio de Ciencia e Innovacion (MCI), Agencia Estatal de Investigacion (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) (Grant No. PGC2018-099857-B-I00), by the Basque Government (Grants Nos. IT1264-19, IT1270-19), by the Fundacion Biofisica Bizkaia and by the Basque Excellence Research Centre (BERC) program of the Basque Government. Dedicated to Professor J. C. Gomez-Fernandez, on occasion of his 70th birthday.es_ES
dc.language.isoenges_ES
dc.publisherNaturees_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/PGC2018-099857-B-I00es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectatomic-force microscopyes_ES
dc.subjectbiophysical propertieses_ES
dc.subjectmitochondrial cholesteroles_ES
dc.subjectsphingomyelinase activityes_ES
dc.subjectmechanical-propertieses_ES
dc.subjectfluorescent-probeses_ES
dc.subjectchain-lengthes_ES
dc.subjectceramidees_ES
dc.subjectbilayerses_ES
dc.subjectdomainses_ES
dc.titleC24:0 and C24:1 sphingolipids in cholesterol-containing, five- and six-component lipid membraneses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-020-71008-8es_ES
dc.identifier.doi10.1038/s41598-020-71008-8
dc.departamentoesBioquímica y biología moleculares_ES
dc.departamentoeuBiokimika eta biologia molekularraes_ES


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This  article  is  licensed  under  a  Creative  Commons  Attribution  4.0  International  License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format,  as  long  as  you  give  appropriate  credit  to  the  original  author(s)  and  the  source,  provide  a  link  to  the  Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material.  If  material  is  not  included  in  the  article’s  Creative  Commons  licence  and  your  intended  use  is  not  permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/