Itemaren erregistro erraza erakusten du

dc.contributor.authorGómez Larrauri, Ana
dc.contributor.authorGangoiti Muñecas, Patricia
dc.contributor.authorPresa Torre, Natalia
dc.contributor.authorDominguez Herrera, Asier
dc.contributor.authorDonati, Chiara
dc.contributor.authorBruni, Paola
dc.contributor.authorTrueba Conde, Miguel Ángel
dc.contributor.authorGómez Muñoz, Antonio
dc.contributor.authorOuro Villasante, Alberto
dc.date.accessioned2021-02-08T11:15:58Z
dc.date.available2021-02-08T11:15:58Z
dc.date.issued2021-02-01
dc.identifier.citationInternational Journal of Molecular Sciences 22 (3) : (2021) // Article ID 1452es_ES
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10810/50091
dc.description.abstractPhosphatidic acid (PA) is a bioactive phospholipid capable of regulating key biological functions, including neutrophil respiratory burst, chemotaxis, or cell growth and differentiation. However, the mechanisms whereby PA exerts these actions are not completely understood. In this work, we show that PA stimulates myoblast proliferation, as determined by measuring the incorporation of [3H]thymidine into DNA and by staining the cells with crystal violet. PA induced the rapid phosphorylation of Akt and ERK1/2, and pretreatment of the cells with specific small interferin RNA (siRNA) to silence the genes encoding these kinases, or with selective pharmacologic inhibitors, blocked PA-stimulated myoblast proliferation. The mitogenic effects of PA were abolished by the preincubation of the myoblasts with pertussis toxin, a Gi protein inhibitor, suggesting the implication of Gi protein-coupled receptors in this action. Although some of the effects of PA have been associated with its possible conversion to lysoPA (LPA), treatment of the myoblasts with PA for up to 60 min did not produce any significant amount of LPA in these cells. Of interest, pharmacological blockade of the LPA receptors 1 and 2, or specific siRNA to silence the genes encoding these receptors, abolished PA-stimulated myoblast proliferation. Moreover, PA was able to compete with LPA for binding to LPA receptors, suggesting that PA can act as a ligand of LPA receptors. It can be concluded that PA stimulates myoblast proliferation through interaction with LPA1 and LPA2 receptors and the subsequent activation of the PI3K/Akt and MEK/ERK1-2 pathways, independently of LPA formation.es_ES
dc.description.sponsorshipThis research was funded by ‘Departamento de Educación del Gobierno Vasco (Gasteiz-Vitoria, Basque Country, Spain) grant number IT-1106-16 and ‘Ministerio de Ciencia, Innovación y Universidades (Madrid, Spain) grant number SAF2016-79695-R.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relationinfo:eu-repo/grantAgreement/MCIU/SAF2016-79695-Res_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/
dc.subjectphosphatidic acides_ES
dc.subjectlysophosphatidic acides_ES
dc.subjectmyoblast proliferationes_ES
dc.subjectlysophosphatidic acid receptorses_ES
dc.titlePhosphatidic Acid Stimulates Myoblast Proliferation through Interaction with LPA1 and LPA2 Receptorses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2021-02-05T14:10:22Z
dc.rights.holder2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).es_ES
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/22/3/1452/htmes_ES
dc.identifier.doi10.3390/ijms22031452
dc.departamentoesBioquímica y biología molecular
dc.departamentoeuBiokimika eta biologia molekularra


Item honetako fitxategiak

Thumbnail

Item hau honako bilduma honetan/hauetan agertzen da

Itemaren erregistro erraza erakusten du

2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Bestelakorik adierazi ezean, itemaren baimena horrela deskribatzen da:2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).