dc.contributor.author | Varela Martínez, Endika | |
dc.contributor.author | Bilbao Arribas, Martín | |
dc.contributor.author | Abendaño Carbajo, Naiara | |
dc.contributor.author | Asín, Javier | |
dc.contributor.author | Pérez, Marta Maria | |
dc.contributor.author | De Andrés, Damián | |
dc.contributor.author | Luján, Lluís | |
dc.contributor.author | Jugo Orrantia, Begoña Marina | |
dc.date.accessioned | 2021-02-24T09:00:21Z | |
dc.date.available | 2021-02-24T09:00:21Z | |
dc.date.issued | 2020-09-17 | |
dc.identifier.citation | Scientific Reports 10(1) : (2020) // Article ID 15240 | es_ES |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10810/50310 | |
dc.description.abstract | Aluminium hydroxide adjuvants are crucial for livestock and human vaccines. Few studies have analysed their effect on the central nervous system in vivo. In this work, lambs received three different treatments of parallel subcutaneous inoculations during 16 months with aluminium-containing commercial vaccines, an equivalent dose of aluminium hydroxide or mock injections. Brain samples were sequenced by RNA-seq and miRNA-seq for the expression analysis of mRNAs, long non-coding RNAs and microRNAs and three expression comparisons were made. Although few differentially expressed genes were identified, some dysregulated genes by aluminium hydroxide alone were linked to neurological functions, the lncRNA TUNA among them, or were enriched in mitochondrial energy metabolism related functions. In the same way, the miRNA expression was mainly disrupted by the adjuvant alone treatment. Some differentially expressed miRNAs had been previously linked to neurological diseases, oxidative stress and apoptosis. In brief, in this study aluminium hydroxide alone altered the transcriptome of the encephalon to a higher degree than commercial vaccines that present a milder effect. The expression changes in the animals inoculated with aluminium hydroxide suggest mitochondrial disfunction. Further research is needed to elucidate to which extent these changes could have pathological consequences. | es_ES |
dc.description.sponsorship | This work was supported by the Spanish Ministry of Economy grant [MINECO project AGL2013-49137-C3 to BMJ, LL and DA]; University of the Basque Country (UPV/EHU) predoctoral fellowships [PIF15/361 to EV-M and PIF17/306 to MB-A]; and University of the Basque Country (UPV/EHU) postdoctoral fellowship [ESPDOC16/43 to NA]. Thanks to M. Ortega for technical help. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/AGL2013-49137-C3 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | long noncoding RNAs | es_ES |
dc.subject | central-nervous-system | es_ES |
dc.subject | oxidative stress | es_ES |
dc.subject | neuronal development | es_ES |
dc.subject | protein families | es_ES |
dc.subject | sequence | es_ES |
dc.subject | genes | es_ES |
dc.subject | model | es_ES |
dc.subject | neurotoxicity | es_ES |
dc.subject | physiology | es_ES |
dc.title | Whole Transcriptome Approach to Evaluate the Effect of Aluminium Hydroxide in Ovine Encephalon | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This article is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-020-71905-y | es_ES |
dc.identifier.doi | 10.1038/s41598-020-71905-y | |
dc.departamentoes | Genética, antropología física y fisiología animal | es_ES |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | es_ES |