Cytocompatibility and Suitability of Protein-Based Biomaterials as Potential Candidates for Corneal Tissue Engineering
dc.contributor.author | Romo Valera, Cristina | |
dc.contributor.author | Guerrero Manso, Pedro Manuel ![]() | |
dc.contributor.author | Arluzea de Jauregizar, Jon Andoni ![]() | |
dc.contributor.author | Echevarría Ecenarro, Jesús ![]() | |
dc.contributor.author | De la Caba Ciriza, María Coro ![]() | |
dc.contributor.author | Andollo Victoriano, María Noelia ![]() | |
dc.date.accessioned | 2021-04-20T10:27:58Z | |
dc.date.available | 2021-04-20T10:27:58Z | |
dc.date.issued | 2021-03-31 | |
dc.identifier.citation | International Journal of Molecular Sciences 22(7) : (2021) // Article ID 3648 | es_ES |
dc.identifier.issn | 1422-0067, | |
dc.identifier.uri | http://hdl.handle.net/10810/51102 | |
dc.description.abstract | The vision impairments suffered by millions of people worldwide and the shortage of corneal donors show the need of substitutes that mimic native tissue to promote cell growth and subsequent tissue regeneration. The current study focused on the in vitro assessment of protein-based biomaterials that could be a potential source for corneal scaffolds. Collagen, soy protein isolate (SPI), and gelatin films cross-linked with lactose or citric acid were prepared and physicochemical, transmittance, and degradation measurements were carried out. In vitro cytotoxicity, cell adhesion, and migration studies were performed with human corneal epithelial (HCE) cells and 3T3 fibroblasts for the films’ cytocompatibility assessment. Transmittance values met the cornea’s needs, and the degradation profile revealed a progressive biomaterials’ decomposition in enzymatic and hydrolytic assays. Cell viability at 72 h was above 70% when exposed to SPI and gelatin films. Live/dead assays and scanning electron microscopy (SEM) analysis demonstrated the adhesion of both cell types to the films, with a similar arrangement to that observed in controls. Besides, both cell lines were able to proliferate and migrate over the films. Without ruling out any material, the appropriate optical and biological properties shown by lactose-crosslinked gelatin film highlight its potential for corneal bioengineering. | es_ES |
dc.description.sponsorship | This research study was supported by grants from the Department of Heath of the Basque Government (RIS3, 2019222049), the University of the Basque Country UPV/EHU-Instituto Clinico Quirurgico de Oftalmologia ICQO (US19/18), and MCI/AEI/FEDER, UE (grant number RTI2018-097100-B-C22). C.R.-V. was supported by a fellowship from the University of the Basque Country UPV/EHU. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MCIU/RTI2018-097100-B-C22 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | corneal scaffold | es_ES |
dc.subject | collagen | es_ES |
dc.subject | gelatin | es_ES |
dc.subject | SPI | es_ES |
dc.subject | cross-linking | es_ES |
dc.subject | cytocompatibility | es_ES |
dc.subject | optical properties | es_ES |
dc.subject | biodegradability | es_ES |
dc.title | Cytocompatibility and Suitability of Protein-Based Biomaterials as Potential Candidates for Corneal Tissue Engineering | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-04-09T13:48:26Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/22/7/3648/htm | es_ES |
dc.identifier.doi | 10.3390/ijms22073648 | |
dc.departamentoes | Biología celular e histología | |
dc.departamentoeu | Zelulen biologia eta histologia |
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Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).