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dc.contributor.authorRuzafa Andrés, Noelia ORCID
dc.contributor.authorPereiro Díez, Xandra ORCID
dc.contributor.authorFonollosa Calduch, Alejandro
dc.contributor.authorAraiz Iribarren, José Javier
dc.contributor.authorAcera Osa, Arantxa
dc.contributor.authorVecino Cordero, Elena ORCID
dc.date.accessioned2021-04-22T07:53:51Z
dc.date.available2021-04-22T07:53:51Z
dc.date.issued2021-03-09
dc.identifier.citationFrontiers In Pharmacology 12 : (2021) // Article ID 606275es_ES
dc.identifier.issn1663-9812
dc.identifier.urihttp://hdl.handle.net/10810/51138
dc.description.abstractPlasma rich in growth factors (PRGF) is a subtype of platelet-rich plasma (PRP) that stimulates tissue regeneration and may promote neuronal survival. It has been employed in ophthalmology to achieve tissue repair in some retinal pathologies, although how PRGF acts in the retina is still poorly understood. As a part of the central nervous system, the retina has limited capacity for repair capacity following damage, and retinal insult can provoke the death of retinal ganglion cells (RGCs), potentially producing irreversible blindness. RGCs are in close contact with glial cells, such as Muller cells, that help maintain homeostasis in the retina. In this study, the aim was to determine whether PRGF can protect RGCs and whether it increases the number of Muller cells. Therefore, PRGF were tested on primary cell cultures of porcine RGCs and Muller cells, as well as on co-cultures of these two cell types. Moreover, the inflammatory component of PRGF was analyzed and the cytokines in the different PRGFs were quantified. In addition, we set out to determine if blocking the inflammatory components of PRGF alters its effect on the cells in culture. The presence of PRGF compromises RGC survival in pure cultures and in co-culture with Muller cells, but this effect was reversed by heat-inactivation of the PRGF. The detrimental effect of PRGF on RGCs could be in part due to the presence of cytokines and specifically, to the presence of pro-inflammatory cytokines that compromise their survival. However, other factors are likely to be present in the PRGF that have a deleterious effect on the RGCs since the exposure to antibodies against these cytokines were insufficient to protect RGCs. Moreover, PRGF promotes Muller cell survival. In conclusion, PRGF hinders the survival of RGCs in the presence or absence of Muller cells, yet it promotes Muller cell survival that could be the reason of retina healing observed in the in vivo treatments, with some cytokines possibly implicated. Although PRGF could stimulate tissue regeneration, further studies should be performed to evaluate the effect of PRGF on neurons and the implication of its potential inflammatory role in such processeses_ES
dc.description.sponsorshipWe acknowledge the support of MINECO-Retos Fondos Fender (RTC-2016-48231), Gobierno Vasco (PUE_2018_1_0004), ELKARTEK (KK-2019/00086), PIBA 2020-1-0026 and MINECO-Retos (PID2019-111139RB-I00) to EVes_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/PID2019-111139RB-I00es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectretinaes_ES
dc.subjectretinal diseasees_ES
dc.subjectPRPes_ES
dc.subjectPRGFes_ES
dc.subjectinflammationes_ES
dc.subjectcytokineses_ES
dc.subjectneurones_ES
dc.subjectgliaes_ES
dc.titlePlasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Muller Glia in Culture but Not the Survival of Retinal Neuronses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://pubmed.ncbi.nlm.nih.gov/33767620/es_ES
dc.identifier.doi10.3389/fphar.2021.606275
dc.departamentoesBiología celular e histologíaes_ES
dc.departamentoesDermatología, oftalmología y otorrinolaringologíaes_ES
dc.departamentoeuDermatologia, oftalmologia eta otorrinolaringologiaes_ES
dc.departamentoeuZelulen biologia eta histologiaes_ES


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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)
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