dc.contributor.author | Padrao, Jorge | |
dc.contributor.author | Ribeiro, Sylvie | |
dc.contributor.author | Lanceros Méndez, Senentxu | |
dc.contributor.author | Rodrigues, Ligia R. | |
dc.contributor.author | Dourado, Fernando | |
dc.date.accessioned | 2021-05-31T08:35:58Z | |
dc.date.available | 2021-05-31T08:35:58Z | |
dc.date.issued | 2020-07 | |
dc.identifier.citation | Heliyon 6(7) : (2020) // Article ID e04372 | es_ES |
dc.identifier.issn | 2405-8440 | |
dc.identifier.uri | http://hdl.handle.net/10810/51673 | |
dc.description.abstract | Bovine lactoferrin (bLF) has been extensively described as a wide spectrum antimicrobial protein. bLF bactericidal activity has been mainly attributed to two different mechanisms: environmental iron depletion and cell membrane destabilization. Due to its antimicrobial properties, bLF has been included in the formulation nutraceutical food products and edible active packages. This work comprises the experimental evidence of the requirement of bLF unrestricted mobility ("free bLF") to effectively perform its bactericidal action. To assess the unrestricted and restricted bLF activity, a nontoxic matrix of bacterial nanocellulose (BNC) was used as carrier, and as an anchoring scaffold, respectively. Therefore, BNC was functionalized with bLF through two different methodologies: (i) bLF was embedded within the three-dimensional structure of BNC and; (ii) bLF was covalently bounded to the nanofibrils of BNC. bLF efficiency was tested against two bacteria isolated from clinical specimens, Escherichia coli and Staphylococcus aureus. bLF concentration after covalent binding to BNC was two-fold higher in comparison to the embedding method. Nevertheless, only the embedded bLF exhibited a significant bactericidal activity, due to bLF ability to permeate the BNC matrix and execute its bactericidal action. | es_ES |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. This work was also supported by the FCT in the framework of the Strategic Funding of UID/FIS/04650/2020 and projects PTDC/BTM-MAT/28237/2017 and PTDC/EMDEMD/28159/2017. The authors acknowledge funding by the Spanish State Research Agency (AEI) and the European Regional Development Fund through the project PID2019-106099RBC43/AEI/10.13039/501100011033 and from the Basque Government Industry and Education Department under the ELKARTEK, HAZITEK and PIBA (PIBA-2018-06) programs, respectively. In addition, J. Padrao (SFRH/BD/64901/2009) and S. Ribeiro (SFRH/BD/111478/2015) would like to acknowledge their doctoral grants awarded by FTC. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | materials science | es_ES |
dc.subject | food technology | es_ES |
dc.subject | microbiology | es_ES |
dc.subject | bacterial nanocellulose | es_ES |
dc.subject | bovine lactoferrin | es_ES |
dc.subject | antibacterial protein activity | es_ES |
dc.title | Effect of Bacterial Nanocellulose Binding on the Bactericidal Activity of Bovine Lactoferrin | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open access article under the CC BY-NC-ND license | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://www-sciencedirect-com.ehu.idm.oclc.org/science/article/pii/S2405844020312160 | es_ES |
dc.identifier.doi | 10.1016/j.heliyon.2020.e04372 | |