Optimization of the Bioactivation of Isoflavones in Soymilk by Lactic Acid Bacteria
dc.contributor.author | Izaguirre Campoverde, Jon Kepa | |
dc.contributor.author | Barañano Orbe, Leire | |
dc.contributor.author | Castañón de la Torre, Sonia | |
dc.contributor.author | Alcorta Calvo, Miren Itziar | |
dc.contributor.author | Quirós, Luis M. | |
dc.contributor.author | Garbisu Crespo, Carlos | |
dc.date.accessioned | 2021-07-09T16:45:10Z | |
dc.date.available | 2021-07-09T16:45:10Z | |
dc.date.issued | 2021-05-28 | |
dc.identifier.citation | Processes 9(6) : (2021) // Article ID 963 | es_ES |
dc.identifier.issn | 2227-9717 | |
dc.identifier.uri | http://hdl.handle.net/10810/52264 | |
dc.description.abstract | Soybeans and soy-based products contain isoflavones which can be used for nutraceutical and medical applications. In soybeans and in unfermented soy foods, isoflavones are normally present as glycosides. Isoflavone glycosides can be enzymatically converted to isoflavone aglycones, thus releasing the sugar molecule. The effective absorption of isoflavones in humans requires the bioconversion of isoflavone glycosides to isoflavone aglycones through the activity of the enzyme β-glucosidase. The objective was to assess the capacity of 42 bacterial strains (belonging to Lactobacillus, Streptococcus and Enterococcus) to produce β-glucosidase activity. The strain that showed the highest β-glucosidase activity (Lactobacillus plantarum 128/2) was then used for the optimization of the bioconversion of genistin and daidzin present in commercial soymilk to their aglycone forms genistein and daidzein. The contribution of process parameters (temperature, inoculum size, time) to the efficiency of such bioactivation was tested. Lactobacillus plantarum 128/2 was able to completely bioactivate soymilk isoflavones under the following conditions: 25 °C temperature, 2% inoculum size and 48 h process time. These results confirm the suitability of lactic acid bacteria for the bioactivation of isoflavones present in soymilk and provide an interesting candidate (L. plantarum 182/2) for food industries to perform this transformation. | es_ES |
dc.description.sponsorship | Jon Kepa Izaguirre was the recipient of a predoctoral fellowship from the Basque Government. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | β-glucosidase | es_ES |
dc.subject | isoflavone glycosides | es_ES |
dc.subject | isoflavone aglycones | es_ES |
dc.subject | nutraceuticals | es_ES |
dc.subject | phytoestrogens | es_ES |
dc.title | Optimization of the Bioactivation of Isoflavones in Soymilk by Lactic Acid Bacteria | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-06-24T14:11:42Z | |
dc.rights.holder | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2227-9717/9/6/963 | es_ES |
dc.identifier.doi | 10.3390/pr9060963 | |
dc.departamentoes | Bioquímica y biología molecular | |
dc.departamentoeu | Biokimika eta biologia molekularra |
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Except where otherwise noted, this item's license is described as © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).