dc.contributor.author | Dong, Pin | |
dc.contributor.author | Stellmacher, Johannes | |
dc.contributor.author | Bouchet, Lydia M. | |
dc.contributor.author | Nieke, Marius | |
dc.contributor.author | Kumar, Amit | |
dc.contributor.author | Osorio Blanco, Ernesto Rafael | |
dc.contributor.author | Nagel, Gregor | |
dc.contributor.author | Lohan, Silke B. | |
dc.contributor.author | Teutloff, Christian | |
dc.contributor.author | Patzelt, Alexa | |
dc.contributor.author | Schäfer-Korting, Monika | |
dc.contributor.author | Calderón, Marcelo | |
dc.contributor.author | Meinke, Martina C. | |
dc.contributor.author | Alexiev, Ulrike | |
dc.date.accessioned | 2021-07-19T10:56:36Z | |
dc.date.available | 2021-07-19T10:56:36Z | |
dc.date.issued | 2021-06-25 | |
dc.identifier.citation | Angewandte Chemie International Edition 60(27) : 14938-14944 (2021) | es_ES |
dc.identifier.issn | 1433-7851 | |
dc.identifier.issn | 1521-3773 | |
dc.identifier.uri | http://hdl.handle.net/10810/52502 | |
dc.description.abstract | Simultaneous visualization and concentration quantification of molecules in biological tissue is an important though challenging goal. The advantages of fluorescence lifetime imaging microscopy (FLIM) for visualization, and electron paramagnetic resonance (EPR) spectroscopy for quantification are complementary. Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label-mediated fluorescence quenching. Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. The DL can easily be coupled to the biomolecule of choice, enabling in vivo and in vitro applications. This novel approach paves the way for elegant studies ranging from fundamental biological investigations to preclinical drug research, as shown in proof-of-principle penetration experiments in human skin ex vivo. | es_ES |
dc.description.sponsorship | This work was financially supported by the German Research Foundation (Collaborative Research Center 1112, Project B01 to P.D., S.B.L., and M.C.M., B03 to U.A., C02 to M.S.K, and A04 to M.C., and partially by Collaborative Research Center 1078, Project A2, and Collaborative Research Center 1449 to U.A.). We would like to acknowledge the assistance of the Core Facility BioSupraMol supported by the DFG and thank Dr. A. Springer. M.C. acknowledges IKERBASQUE-Basque Foundation for Science and MINECO project RTI2018-099227-B-I00 for financial support. E.R.O.-B. gratefully thanks the Dahlem Research School for a scholarship. P.D. thanks the China Scholarship Council for financial support. Open access funding enabled and organized by Projekt DEAL | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/RTI2018-099227-B-I00 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | 3-carboxy-proxyl (PCA) | es_ES |
dc.subject | fluorescence | es_ES |
dc.subject | multiplexed FLIM– | es_ES |
dc.subject | EPR spectroscopy | es_ES |
dc.subject | Rhodamine  | es_ES |
dc.subject | B | es_ES |
dc.subject | spin labels | es_ES |
dc.subject | penetration properties | es_ES |
dc.subject | skin penetration | es_ES |
dc.subject | barrier function | es_ES |
dc.subject | rhodamine-B | es_ES |
dc.subject | in-vitro | es_ES |
dc.subject | nanoparticles | es_ES |
dc.subject | dexamethasone | es_ES |
dc.subject | technologies | es_ES |
dc.subject | nanocarriers | es_ES |
dc.subject | fluorophore | es_ES |
dc.title | A Dual Fluorescence-Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM-EPR Spectroscopy | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY-NC-ND 4.0) | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/10.1002/anie.202012852 | es_ES |
dc.identifier.doi | 10.1002/anie.202012852 | |