In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies
dc.contributor.author | Atabakhshi-Kashi, Mona | |
dc.contributor.author | Carril García, Mónica | |
dc.contributor.author | Mahdavi, Hossein | |
dc.contributor.author | Parak, Wolfgang J. | |
dc.contributor.author | Carrillo Carrión, Carolina | |
dc.contributor.author | Khajeh, Khosro | |
dc.date.accessioned | 2021-09-13T08:48:30Z | |
dc.date.available | 2021-09-13T08:48:30Z | |
dc.date.issued | 2021-07-24 | |
dc.identifier.citation | Nanomaterials 11(8) : (2021) // Article ID 1906 | es_ES |
dc.identifier.issn | 2079-4991 | |
dc.identifier.uri | http://hdl.handle.net/10810/53072 | |
dc.description.abstract | Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine–fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly). | es_ES |
dc.description.sponsorship | This research was financially supported by the research core of TMU (Grant No: IG-39707) and the Biotechnology Development Council of the Islamic Republic of Iran (Grant No: 950709). Part of this work was funded by the Cluster of Excellence ‘Advanced Imaging of Matter’ of the Deutsche Forschungsgemeinschaft (DFG)-EXC 2056-project ID 390715994. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | nanoparticles | es_ES |
dc.subject | quantum dots | es_ES |
dc.subject | antibody conjugation | es_ES |
dc.subject | fluorination | es_ES |
dc.subject | self-assembly | es_ES |
dc.title | In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-09-09T13:44:23Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2079-4991/11/8/1906/htm | es_ES |
dc.identifier.doi | 10.3390/nano11081906 | |
dc.departamentoes | Bioquímica y biología molecular | |
dc.departamentoeu | Biokimika eta biologia molekularra |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).