dc.contributor.author | González Pujana, Ainhoa | |
dc.contributor.author | Beloqui García, Ana | |
dc.contributor.author | Aguirre Anda, José Javier | |
dc.contributor.author | Igartua Olaechea, Manuela ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Santos Vizcaíno, Edorta ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Hernández Martín, Rosa María ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.date.accessioned | 2022-05-17T08:58:57Z | |
dc.date.available | 2022-05-17T08:58:57Z | |
dc.date.issued | 2022-03 | |
dc.identifier.citation | European Journal of Pharmaceutics and Biopharmaceutics 172 : 31-40 (2022) | es_ES |
dc.identifier.issn | 0939-6411 | |
dc.identifier.issn | 1873-3441 | |
dc.identifier.uri | http://hdl.handle.net/10810/56563 | |
dc.description.abstract | [EN] The ability of mesenchymal stromal cells (MSCs) to release a plethora of immunomodulatory factors makes them valuable candidates to overcome inflammatory bowel diseases (IBD). However, this cell therapy approach is still limited by major issues derived from nude MSC-administration, including a rapid loss of their immunomodulatory phenotype that impairs factor secretion, low persistence and impossibility to retrieve the cells in case of adverse effects. Here, we designed a licensing hydrogel system to address these limitations and thus, obtain a continuous delivery of bioactive factors. IFN gamma-loaded heparin-coated beads were included in injectable in situ crosslinking alginate hydrogels, providing a 3D microenvironment that ensured continuous inflammatory licensing, cell persistence and implant retrievability. Licensing-hydrogel encapsulated human MSCs (hMSCs) were subcutaneously xenotransplanted in an acute mouse model of ulcerative colitis. Results showed that encapsulated hMSCs exerted a delocalized systemic protection, not presenting significant differences to healthy mice in the disease activity index, colon weight/length ratio and histological score. At day 7, cells were easily retrieved and ex vivo assays showed fully viable hMSCs that retained an immunomodulatory phenotype, as they continued secreting factors including PGE2 and Gal-9. Our data demonstrate the capacity of licensing hydrogelencapsulated hMSCs to limit the in vivo progression of IBD. | es_ES |
dc.description.sponsorship | Authors thank Ministerio de Economia, Industria y Competitividad (SAF2017-82292-R, MINECO/AEI/FEDER, UE) and Eusko Jaurlaritza (Grupos Consolidados, No ref: IT907-16) and ICTS "NANBIOSIS" (Drug Formulation Unit, U10). A. Beloqui is a research associate from the Belgian National Funds of Research (F.R.S.-FNRS). A. Gonzalez-Pujana thanks the University of the Basque Country (UPV/EHU) for the postdoctoral grant (ESPDOC20/119). Open Access funding provided by University of Basque Country (UPV/EHU). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2017-82292-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | mesenchymal stromal cell (MSC) | es_ES |
dc.subject | Imimunomodulation | es_ES |
dc.subject | inflammatory bowel diseases (IBD) | es_ES |
dc.subject | inflammatory licensing | es_ES |
dc.subject | hydrogel | es_ES |
dc.subject | subcutaneous delivery | es_ES |
dc.title | Mesenchymal stromal cells encapsulated in licensing hydrogels exert delocalized systemic protection against ulcerative colitis via subcutaneous xenotransplantation | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0939641122000133?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.ejpb.2022.01.007 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | es_ES |
dc.departamentoeu | Farmazia eta elikagaien zientziak | es_ES |