In vitro and in vivo anti-Candida activity of citral in combination with fluconazole
dc.contributor.author | Miranda Cadena, Katherine | |
dc.contributor.author | Marcos Arias, Cristina | |
dc.contributor.author | Pérez Rodríguez, Aitzol ![]() | |
dc.contributor.author | Cabello Beitia, Iván | |
dc.contributor.author | Mateo Alesanco, Estibaliz ![]() | |
dc.contributor.author | Sevillano Peña, Elena | |
dc.contributor.author | Madariaga Torres, Lucila ![]() | |
dc.contributor.author | Quindós Andrés, Guillermo | |
dc.contributor.author | Eraso Barrio, María Elena ![]() | |
dc.date.accessioned | 2022-05-26T07:49:30Z | |
dc.date.available | 2022-05-26T07:49:30Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Journal of Oral Microbiology 14(1) : (2022) // Article ID 2045813 | es_ES |
dc.identifier.issn | 2000-2297 | |
dc.identifier.uri | http://hdl.handle.net/10810/56734 | |
dc.description.abstract | [EN] Background The ability of Candida to develop biofilms on inert surfaces or living tissues favors recalcitrant and chronic candidiasis associated, in many instances, with resistance to current antifungal therapy. Aim The aim of this study was to evaluate the antifungal activity of citral, a phytocompound present in lemongrass essential oil, in monotherapy and combined with fluconazole against azole-resistant Candida planktonic cells and biofilms. The effect of citral combined with fluconazole was also analysed with regard to the expression of fluconazole resistance-associated genes in Candida albicans and the effectiveness of the combination therapy in a Caenorhabditis elegans model of candidiasis. Results Citral reduced biofilm formation at initial stages and the metabolic activity of the mature biofilm. The combination of citral with fluconazole was synergistic, with a significant increase in the survival of C. elegans infected with Candida. RNA analysis revealed a reduction of the expression of the efflux pump encoded by MDR1, leading to a greater effect of fluconazole. Conclusion Citral in monotherapy and in combination with fluconazole could represent an interesting therapy to treat recalcitrant Candida infections associated to biofilms. | es_ES |
dc.description.sponsorship | This research was supported by Gobierno Vasco-Eusko Jaurlaritza [Eusko Jaurlaritza GIC15/78 IT-990-16] and by Fundacion ONCE "Oportunidad al Talento" and Fondo Social Europeo CM-A [C.M.-A.]; Ministerio de Economia, Industria y Competitividad, Gobierno de Espan [PID2020-117983RB-I00]. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Taylor & Francis | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2020-117983RB-I00 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/es/ | * |
dc.subject | candida | es_ES |
dc.subject | biofilm | es_ES |
dc.subject | citral | es_ES |
dc.subject | azole resistance | es_ES |
dc.subject | caenorhabditis elegans | es_ES |
dc.subject | gene expression | es_ES |
dc.title | In vitro and in vivo anti-Candida activity of citral in combination with fluconazole | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | es_ES |
dc.rights.holder | Atribución-NoComercial 3.0 España | * |
dc.relation.publisherversion | https://www.tandfonline.com/doi/full/10.1080/20002297.2022.2045813 | es_ES |
dc.identifier.doi | 10.1080/20002297.2022.2045813 | |
dc.departamentoes | Inmunología, microbiología y parasitología | es_ES |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | es_ES |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.