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dc.contributor.authorRomayor Arredondo, Irene ORCID
dc.contributor.authorHerrera, Lara ORCID
dc.contributor.authorBurón, María ORCID
dc.contributor.authorMartín Inaraja, Myriam
dc.contributor.authorPrieto López, Laura
dc.contributor.authorEtxaniz Díaz de Durana, Jone
dc.contributor.authorInglés Ferrándiz, Marta
dc.contributor.authorPineda Martí, José Ramón ORCID
dc.contributor.authorEguizabal Argaiz, Cristina ORCID
dc.date.accessioned2022-08-02T08:58:17Z
dc.date.available2022-08-02T08:58:17Z
dc.date.issued2022
dc.identifier.citationBiomedicines 10(6) : (2022) // Article ID 1358es_ES
dc.identifier.issn2227-9059
dc.identifier.urihttp://hdl.handle.net/10810/57143
dc.description.abstractThe successful reprogramming of human somatic cells into induced pluripotent stem cells (hiPSCs) represented a turning point in the stem cell research field, owing to their ability to differentiate into any cell type with fewer ethical issues than human embryonic stem cells (hESCs). In mice, PSCs are thought to exist in a naive state, the cell culture equivalent of the immature pre-implantation embryo, whereas in humans, PSCs are in a primed state, which is a more committed pluripotent state than a naive state. Recent studies have focused on capturing a similar cell stage in human cells. Given their earlier developmental stage and therefore lack of cell-of-origin epigenetic memory, these cells would be better candidates for further re-differentiation, use in disease modeling, regenerative medicine and drug discovery. In this study, we used primed hiPSCs and hESCs to evaluate the successful establishment and maintenance of a naive cell stage using three different naive-conversion media, both in the feeder and feeder-free cells conditions. In addition, we compared the directed differentiation capacity of primed and naive cells into the three germ layers and characterized these different cell stages with commonly used pluripotent and lineage-specific markers. Our results show that, in general, naive culture NHSM medium (in both feeder and feeder-free systems) confers greater hiPSCs and hESCs viability and the highest naive pluripotency markers expression. This medium also allows better cell differentiation cells toward endoderm and mesoderm.es_ES
dc.description.sponsorshipThis work was supported by the Health Department of the Basque Government (Grant 2019111068, 2019/4703, 2020111058, 2020333032, 2021333057 and 2021333012), Merck-Salud Founda- tion (FSALUD17/004), Economic Development and Infrastructures Department of the Basque Govern- ment (KK-2020/00068), EITB Maratoia (BIO21/COV/030), Project “PI18/01299” and “PI21/01187”, funded by Instituto de Salud Carlos III and co-funded by European Union (ERDF) “A way to make Europe”, “ICI21/00095” funded by Instituto de Salud Carlos III and co-funded by European Union (NextGenerationEU), “Plan de Recuperación Transformación y Resiliencia” Investigación Clínica Independiente 2021–Acción Estratégica Salud 2017–2020, RICORS: (RD21/00017/0024) Red Española de Terapias Avanzadas TERAV ISCIII. Funded by Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (NextGenerationEU) “Plan de Recuperación Transformación y Resiliencia” Redes de Investigación Cooperativa Orientadas a Resultados en Salud (RICORS) 2021–Acción Estratégica Salud 2017–2020. L.H. was supported by the Jesus Gangoiti Barrera Foundation and the Asociación Española contra el Cáncer (AECC) AECC16/501 and the Fundación Mutua Madrileña AP176182020. M.M-I was supported by Jesus Gangoiti Barrera Foundation. I.R was supported by Margarita Salas Grant “MARSA21/60” and the Jesus Gangoiti Barrera Foundation. M.I-F. was supported by Inocente Inocente Foundation FII18/003. J.R.P. has grant “RYC-2013-13450” funded by MCIN/AEI/10.13039/501100011033, by the European Social Fund “ESF investing in your future”.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/RYC-2013-13450es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjecthiPSCses_ES
dc.subjecthESCses_ES
dc.subjectpluripotencyes_ES
dc.subjectnaive statuses_ES
dc.subjectprimed statuses_ES
dc.subjectcell differentiationes_ES
dc.subjectectodermes_ES
dc.subjectmesodermes_ES
dc.subjectendodermes_ES
dc.titleA Comparative Study of Cell Culture Conditions during Conversion from Primed to Naive Human Pluripotent Stem Cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2022-06-23T12:20:59Z
dc.rights.holder© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)es_ES
dc.relation.publisherversionhttps://www.mdpi.com/2227-9059/10/6/1358es_ES
dc.identifier.doi10.3390/biomedicines10061358
dc.departamentoesBiología celular e histología
dc.departamentoeuZelulen biologia eta histologia


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© 2022 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license (https://
creativecommons.org/licenses/by/
4.0/)
Except where otherwise noted, this item's license is described as © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)