MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker
dc.contributor.author | Abrisqueta Gonzalez, Marta | |
dc.contributor.author | Cerdido, Sonia | |
dc.contributor.author | Sánchez Beltrán, José | |
dc.contributor.author | Martínez Vicente, Idoya | |
dc.contributor.author | Herraiz, Cecilia | |
dc.contributor.author | Lambertos, Ana | |
dc.contributor.author | Olivares, Conchi | |
dc.contributor.author | Sevilla Mambrilla, Arrate | |
dc.contributor.author | Alonso Alegre, Santos | |
dc.contributor.author | Boyano López, María Dolores | |
dc.contributor.author | García Borrón, José Carlos | |
dc.contributor.author | Jiménez-Cervantes, Celia | |
dc.date.accessioned | 2022-08-31T09:35:49Z | |
dc.date.available | 2022-08-31T09:35:49Z | |
dc.date.issued | 2022-07-26 | |
dc.identifier.citation | Life 12(8) : (2022) // Article ID 1118 | es_ES |
dc.identifier.issn | 2075-1729 | |
dc.identifier.uri | http://hdl.handle.net/10810/57373 | |
dc.description.abstract | Mahogunin Ring Finger 1 (MGRN1), a ubiquitin ligase expressed in melanocytes, interacts with the α melanocyte-stimulating hormone receptor, a well-known melanoma susceptibility gene. Previous studies showed that MGRN1 modulates the phenotype of mouse melanocytes and melanoma cells, with effects on pigmentation, shape, and motility. Moreover, MGRN1 knockdown augmented the burden of DNA breaks in mouse cells, indicating that loss of MGRN1 promoted genomic instability. However, data concerning the roles of MGRN1 in human melanoma cells remain scarce. We analyzed MGRN1 knockdown in human melanoma cells. Transient MGRN1 depletion with siRNA or permanent knockdown in human melanoma cells by CRISPR/Cas9 caused an apparently MITF-independent switch to a more dendritic phenotype. Lack of MGRN1 also increased the fraction of human cells in the S phase of the cell cycle and the burden of DNA breaks but did not significantly impair proliferation. Moreover, in silico analysis of publicly available melanoma datasets and estimation of MGRN1 in a cohort of clinical specimens provided preliminary evidence that MGRN1 expression is higher in human melanomas than in normal skin or nevi and pointed to an inverse correlation of MGRN1 expression in human melanoma with patient survival, thus suggesting potential use of MGRN1 as a melanoma biomarker. | es_ES |
dc.description.sponsorship | This research was funded by grant SAF2018_RTI2018-094929-B-I00 financed by FEDER/Ministerio de Ciencia e Innovación—Agencia Estatal de Investigación (Spain) (to C.J.-C. and J.C.G.-B.), and by grant UPV/EHU GIU20/035 (to S.A and M.D.B.). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Mahogunin Ring Finger 1 (MGRN1) | es_ES |
dc.subject | melanocytes | es_ES |
dc.subject | melanoma | es_ES |
dc.subject | DNA damage | es_ES |
dc.subject | biomarker | es_ES |
dc.title | MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2022-08-25T11:18:49Z | |
dc.rights.holder | © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2075-1729/12/8/1118 | es_ES |
dc.identifier.doi | 10.3390/life12081118 | |
dc.departamentoes | Genética, antropología física y fisiología animal | |
dc.departamentoes | Biología celular e histología | |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | |
dc.departamentoeu | Zelulen biologia eta histologia |
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Except where otherwise noted, this item's license is described as © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).