dc.contributor.author | Brito Pereira, Ricardo | |
dc.contributor.author | Macedo, André Gustavo Silva | |
dc.contributor.author | Ribeiro, Clarisse | |
dc.contributor.author | Cardoso, Vanessa F. | |
dc.contributor.author | Lanceros Méndez, Senentxu | |
dc.date.accessioned | 2022-09-08T11:24:45Z | |
dc.date.available | 2022-09-08T11:24:45Z | |
dc.date.issued | 2022-08 | |
dc.identifier.citation | Applied Materials Today 28 : (2022) // Article ID 101507 | es_ES |
dc.identifier.issn | 2352-9407 | |
dc.identifier.uri | http://hdl.handle.net/10810/57658 | |
dc.description.abstract | [EN] Portable analytical systems are versatile tools for application in areas including biomedicine, biosecurity, food safety and environmental monitoring. This work contributes to the increasing demand for low-cost, environmentally friendly substrates for portable analytical systems by using natural Bombyx mori cocoons. Further, silk fibroin is also extracted from these cocoons and electrospun into oriented and randomly oriented fiber substrates. Oxygen plasma treatment is applied to improve their hydrophilicity. Fiber morphology, mechanical properties, porosity, thermal characteristics and surface contact angle are extensively characterized and the ability of the samples for passive capillary flows demonstrated. Plasma treated pressed cocoons show superhydrophilicity, capillary flow rates of 44.8 +/- 3.75 mm.min-1, and high mechanical resistance with Young's modulus values up to 592.13 +/- 19.83 MPa. The developed materials are used as substrates for the colorimetric quantification of three commonly scrutinized clinical analytes. Hydrophobic barriers are first wax-printed on all samples with a proper design and albumin assays are performed on all substrates. Further assays for uric acid and glucose quantification are successfully accomplished on the pressed cocoons after a simple in between washing step, with overall high coefficient of determination, proving the suitability of the developed materials as low-cost, sustainable and reusable microfluidic substrates. | es_ES |
dc.description.sponsorship | This work was supported by the Portuguese Foundation for Science and Technology (FCT) under strategic funding UID/FIS/04650/2020, UIDB/04436/2020, UIDP/04436/2020 and project PTDC/EMD-EMD/28159/2017 (POCI-01-0145-FEDER-028159) . The authors also thank FCT for financial support under grants SFRH/BD/140698/2018 (R.B. P.) , 2020.09218.BD (A.S.M.) , 2020.04163.CEECIND (C.R.) and 2020.02304.CEECIND (V.F.C.) . Finally, the authors acknowledge funding by Spanish State Research Agency (AEI) and the European Regional Development Fund (ERFD) through the project PID2019-106099RB-C43/AEI/10.13039/501100011033 and from the Basque Government Industry Departments under the ELKARTEK program. Finally, the au-thors also thank Dr. J. Borges and Prof. F. Vaz for experimental support. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2019-106099RB-C43 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | microfluidic | es_ES |
dc.subject | point of care | es_ES |
dc.subject | portable analytical devices | es_ES |
dc.subject | silk | es_ES |
dc.subject | sustainability | es_ES |
dc.title | Natural based reusable materials for microfluidic substrates: The silk road towards sustainable portable analytical systems | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S2352940722001421?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.apmt.2022.101507 | |