dc.contributor.author | Simoes, Anabel R. | |
dc.contributor.author | Neto, Marta | |
dc.contributor.author | Alves, Carolina S. | |
dc.contributor.author | Santos, Mariana B. | |
dc.contributor.author | Fernández Hernández, Ismael | |
dc.contributor.author | Veiga Fernandes, Henrique | |
dc.contributor.author | Brea, David | |
dc.contributor.author | Durá, Irene | |
dc.contributor.author | Encinas Pérez, Juan Manuel | |
dc.contributor.author | Rhiner, Christa | |
dc.date.accessioned | 2022-09-12T18:15:47Z | |
dc.date.available | 2022-09-12T18:15:47Z | |
dc.date.issued | 2022-07 | |
dc.identifier.citation | Developmental Cell 57(13) : 1661-1675 (2022) | es_ES |
dc.identifier.issn | 1534-5807 | |
dc.identifier.issn | 1878-1551 | |
dc.identifier.uri | http://hdl.handle.net/10810/57704 | |
dc.description.abstract | [EN] Recruitment of stem cells is crucial for tissue repair. Although stem cell niches can provide important signals, little is known about mechanisms that coordinate the engagement of disseminated stem cells across an injured tissue. In Drosophila, adult brain lesions trigger local recruitment of scattered dormant neural stem cells suggesting a mechanism for creating a transient stem cell activation zone. Here, we find that injury trig-gers a coordinated response in neuro-glial clusters that promotes the spread of a neuron-derived stem cell factor via glial secretion of the lipocalin-like transporter Swim. Strikingly, swim is induced in a Hif1-a-depen-dent manner in response to brain hypoxia. Mammalian Swim (Lcn7) is also upregulated in glia of the mouse hippocampus upon brain injury. Our results identify a central role of neuro-glial clusters in promoting neural stem cell activation at a distance, suggesting a conserved function of the HIF1-a/Swim/Wnt module in con-necting injury-sensing and regenerative outcomes. | es_ES |
dc.description.sponsorship | We thank the Bloomington Stock Center, the VDRC and the DSHB for antibodies and fly stocks; Christian Bokel and Alisson Gontijo for advice with CRISPR; and Stefan Thor, Claude Desplan, Pablo Wappner, Andrea PageMcCaw, Jean-Paul Vincent, Yuh Nung Jan, Gine ' s Morata, Christian Lehner, Julia Cordero, Boris Egger, and Marc Freeman for sharing antibodies or fly stocks. We thank Eduardo Moreno for support with microarrays; Esha Madan and Boris Egger for advice with hypoxia assays; Raquel Toma ' s for help with target vector design and cloning; Carmo Soares for technical assistance; Maria Diaz de la Loza for graphical support; and Carlos Ribeiro for valuable discussions on the manuscript. This work has been supported by the Champalimaud Foundation, a Fundacao para a Ciencia e Tecnologia (FCT) research grant to C.R. (PTDC/BIA-MOL/31170/2017), an FCT PhD fellowship to A.R.S. (SFRH/BD/121028/2016), an FPI (MINECO) predoctoral fellowship to I.D., a MINECO PCIN-2016-128 (ERA-NET-NEURON III program) and SAF2015-70866-R (with FEDER funds) to J.M.E, an FCT Scientific Employment Stimulus-2018 and PTDC/MED-IMU/0870/2020 to D.B. and grants from the European Research Council, Paul G. Allen Frontiers Group and Fundacion la Caixa to H.V.-F., plus the infrastructure support from CONGENTO (project LISBOA-01-0145-FEDER-022170). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/PCIN-2016-128 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2015-70866-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | quiescent neural stem cells | es_ES |
dc.subject | damage responsive | es_ES |
dc.subject | Drosophila | es_ES |
dc.subject | neuro-glial cooperation | es_ES |
dc.subject | brain injury | es_ES |
dc.subject | HIF1-α | es_ES |
dc.subject | Swim/Lcn7 | es_ES |
dc.subject | hypoxia | es_ES |
dc.title | Damage-responsive neuro-glial clusters coordinate the recruitment of dormant neural stem cells in Drosophila | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S1534580722003720?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.devcel.2022.05.015 | |
dc.departamentoes | Neurociencias | es_ES |
dc.departamentoeu | Neurozientziak | es_ES |