Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis.
dc.contributor.author | Quintanilla, Isabel | |
dc.contributor.author | Jung, Gerhard | |
dc.contributor.author | Jimeno, Mireya | |
dc.contributor.author | Lozano, Juan José | |
dc.contributor.author | Sidorova, Julia | |
dc.contributor.author | Camps, Jordi | |
dc.contributor.author | Carballal, Sabela | |
dc.contributor.author | Bujanda Fernández de Pierola, Luis ![]() | |
dc.contributor.author | Vera, María Isabel | |
dc.contributor.author | Quintero, Enrique | |
dc.contributor.author | Carrillo Palau, Marta | |
dc.contributor.author | Cuatrecasas, Miriam | |
dc.contributor.author | Castells, Antoni | |
dc.contributor.author | Panés, Julià | |
dc.contributor.author | Ricart, Elena | |
dc.contributor.author | Moreira, Leticia | |
dc.contributor.author | Balaguer, Francesc | |
dc.contributor.author | Pellisé, María | |
dc.date.accessioned | 2022-10-03T16:51:41Z | |
dc.date.available | 2022-10-03T16:51:41Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Clinical and Translational Gastroenterology 13(7) : (2022) // Article ID e00489 | es_ES |
dc.identifier.issn | 2155-384X | |
dc.identifier.uri | http://hdl.handle.net/10810/57900 | |
dc.description.abstract | INTRODUCTION: Colorectal cancer (CRC) is a potentially life-threatening complication of long-standing ulcerative colitis (UC). MicroRNAs (miRNA) are epigenetic regulators that have been involved in the development of UC-associated CRC. However, their role as potential mucosal biomarkers of neoplastic progression has not been adequately studied. METHODS: In this study, we analyzed the expression of 96 preselected miRNAs in human formalin-fixed and paraffin-embedded tissue of 52 case biopsies (20 normal mucosa, 20 dysplasia, and 12 UC-associated CRCs) and 50 control biopsies (10 normal mucosa, 21 sporadic adenomas, and 19 sporadic CRCs) by using Custom TaqMan Array Cards. For validation of deregulated miRNAs, we performed individual quantitative real-time polymerase chain reaction in an independent cohort of 50 cases (13 normal mucosa, 25 dysplasia, and 12 UC-associated CRCs) and 46 controls (7 normal mucosa, 19 sporadic adenomas, and 20 sporadic CRCs). RESULTS: Sixty-four miRNAs were found to be differentially deregulated in the UC-associated CRC sequence. Eight of these miRNAs were chosen for further validation. We confirmed miR-31, -106a, and -135b to be significantly deregulated between normal mucosa and dysplasia, as well as across the UC-associated CRC sequence (all P < 0.01). Notably, these miRNAs also confirmed to have a significant differential expression compared with sporadic CRC (all P < 0.05). DISCUSSION: UC-associated and sporadic CRCs have distinct miRNA expression patterns, and some miRNAs indicate early neoplastic progression. | es_ES |
dc.description.sponsorship | This work was funded by grants from the Instituto de Salud Carlos III (PI12/01481; PI19/01050). Project PI19/ 01050 is funded by Instituto de Salud Carlos III (ISCIII) and co funded by the European Union. CIBEREHD is funded by the Insti tuto de Salud Carlos III and Beca Marató de TV3 (201932-30). Parts of this work were also supported by the Xarxa de Bancs de Tumors de Catalunya sponsored by Pla Director d’Oncología de Catalunya (XBTC) and by the Hospital Clínics Premi Fi de Residència (G.J). None of the funding parties has been involved in collection, analysis, and interpretation of the data. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wolters Kluwer | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.title | Differentially Deregulated MicroRNAs as Novel Biomarkers for Neoplastic Progression in Ulcerative Colitis. | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology. This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work pro vided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://journals.lww.com/ctg/Fulltext/2022/07000/Differentially_Deregulated_MicroRNAs_as_Novel.5.aspx | es_ES |
dc.identifier.doi | 10.14309/ctg.0000000000000489 | |
dc.departamentoes | Medicina | es_ES |
dc.departamentoeu | Medikuntza | es_ES |
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Except where otherwise noted, this item's license is described as © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology.
This is an open access article distributed under the terms of the
Creative Commons Attribution-Non Commercial-No Derivatives License 4.0
(CCBY-NC-ND), where it is permissible to download and share the work pro
vided it is properly cited. The work cannot be changed in any way or used
commercially without permission from the journal.