Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors
dc.contributor.author | Etxeberria, Leire | |
dc.contributor.author | Messelmani, Taha | |
dc.contributor.author | Badiola, Jon Haitz | |
dc.contributor.author | Llobera, Andreu | |
dc.contributor.author | Fernández, Luis | |
dc.contributor.author | Vilas Vilela, José Luis | |
dc.contributor.author | Leclerc, Eric | |
dc.contributor.author | Legallais, Cécile | |
dc.contributor.author | Jellali, Rachid | |
dc.contributor.author | Zaldua Huici, Ane Miren | |
dc.date.accessioned | 2022-11-16T17:57:56Z | |
dc.date.available | 2022-11-16T17:57:56Z | |
dc.date.issued | 2022-10-22 | |
dc.identifier.citation | Polymers 14(21) : (2022) // Article ID 4478 | es_ES |
dc.identifier.issn | 2073-4360 | |
dc.identifier.uri | http://hdl.handle.net/10810/58373 | |
dc.description.abstract | Organ-on-chip (OoC) technology is one of the most promising in vitro tools to replace the traditional animal experiment-based paradigms of risk assessment. However, the use of OoC in drug discovery and toxicity studies remain still limited by the low capacity for high-throughput production and the incompatibility with standard laboratory equipment. Moreover, polydimethylsiloxanes, the material of choice for OoC, has several drawbacks, particularly the high absorption of drugs and chemicals. In this work, we report the development of a microfluidic device, using a process adapted for mass production, to culture liver cell line in dynamic conditions. The device, made of cyclic olefin copolymers, was manufactured by injection moulding and integrates Luer lock connectors compatible with standard medical and laboratory instruments. Then, the COC device was used for culturing HepG2/C3a cells. The functionality and behaviour of cultures were assessed by albumin secretion, cell proliferation, viability and actin cytoskeleton development. The cells in COC device proliferated well and remained functional for 9 days of culture. Furthermore, HepG2/C3a cells in the COC biochips showed similar behaviour to cells in PDMS biochips. The present study provides a proof-of-concept for the use of COC biochip in liver cells culture and illustrate their potential to develop OoC. | es_ES |
dc.description.sponsorship | This research was funded by Basque Country Government within the frame of the project BIKAINTEK 2018 (48-AF-W2-2018-00006). Taha Messelmani PhD is funded by ANR (Agence National de la Recherche, France, MIMLIVEROnChip ANR-19-CE19-0020-01 project). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | cyclic olefin copolymer | es_ES |
dc.subject | injection moulding | es_ES |
dc.subject | organ-on-chip | es_ES |
dc.subject | liver | es_ES |
dc.subject | cell growth | es_ES |
dc.title | Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2022-11-10T14:27:56Z | |
dc.rights.holder | © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2073-4360/14/21/4478 | es_ES |
dc.identifier.doi | doi.org/10.3390/polym14214478 | |
dc.departamentoes | Química física | |
dc.departamentoeu | Kimika fisikoa |
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Except where otherwise noted, this item's license is described as © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).