The P. aeruginosa effector Tse5 forms membrane pores disrupting the membrane potential of intoxicated bacteria
dc.contributor.author | González Magaña, Amaia | |
dc.contributor.author | Altuna Álvarez, Jon | |
dc.contributor.author | Queralt Martín, María | |
dc.contributor.author | Largo, Eneko | |
dc.contributor.author | Velázquez Álvarez, Carmen | |
dc.contributor.author | Montánchez Alonso, Itxaso ![]() | |
dc.contributor.author | Bernal, Patricia | |
dc.contributor.author | Alcaraz, Antonio | |
dc.contributor.author | Albesa Jové, David | |
dc.date.accessioned | 2022-11-23T18:27:09Z | |
dc.date.available | 2022-11-23T18:27:09Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Communications Biology 5 : (2022) // Article ID 1189 | es_ES |
dc.identifier.issn | 2399-3642 | |
dc.identifier.uri | http://hdl.handle.net/10810/58521 | |
dc.description.abstract | The type VI secretion system (T6SS) of Pseudomonas aeruginosa injects effector proteins into neighbouring competitors and host cells, providing a fitness advantage that allows this opportunistic nosocomial pathogen to persist and prevail during the onset of infections. However, despite the high clinical relevance of P. aeruginosa, the identity and mode of action of most P. aeruginosa T6SS-dependent effectors remain to be discovered. Here, we report the molecular mechanism of Tse5-CT, the toxic auto-proteolytic product of the P. aeruginosa T6SS exported effector Tse5. Our results demonstrate that Tse5-CT is a pore-forming toxin that can transport ions across the membrane, causing membrane depolarisation and bacterial death. The membrane potential regulates a wide range of essential cellular functions; therefore, membrane depolarisation is an efficient strategy to compete with other microorganisms in polymicrobial environments. | es_ES |
dc.description.sponsorship | We gratefully acknowledge the Laboratories of Dr. Daniel Ladant (Institut Pasteur, Paris) and Dr. Victor de Lorenzo (Centro Nacional de Biotecnologia, Madrid) for the plasmids received (pKTop and pSEVA plasmids, respectively). Also, we would like to acknowledge the Laboratory of Dr. Joseph Mougous for the P. aeruginosa strains received. The technical assistance from Cristina Civantos and Adrian Ruiz is also very much appreciated. We acknowledge the FGCZ for the mass spectrometry analyses and the technical support (Functional Genomics Center Zurich (FGCZ), University/ETH Zurich). D.A.-J. acknowledges support by the MINECO Contracts CTQ2016-76941-R and PID2021-127816NB-I00, Fundacion Biofisica Bizkaia, the Basque Excellence Research Centre (BERC) programme, and IT709-13 and IT1745-22 of the Basque Government, and Fundacion BBVA. A.G.-M. acknowledges the financial support received from the Spanish Ministry of Universities and the Grants for the requalification of the Spanish university system for 2021-2023, financed by the European Union-Next Generation EU-Margarita Salas Modality. A.A. acknowledges support from the Spanish Ministry of Science and Innovation (Project 2019-108434GB-I00 funded by MCIN/AEI/10.13039/501100011033), Generalitat Valenciana (project AICO/2020/066) and Universitat Jaume I (project UJI-B2018-53). M.Q.-M. acknowledges support from the Spanish Ministry of Science and Innovation (Project IJC2018-035283-I funded by MCIN/AEI/10.13039/501100011033) and Universitat Jaume I (project UJI-A2020-21). P.B acknowledges the financial support received from the Spanish Ministry of Science and Innovation through the Ramon y Cajal Programme (contract RYC2019-026551-I). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2021-127816NB-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2019-108434GB-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/CTQ2016-76941-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICIU/IJC2018-035283-I | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/RYC2019-026551-I | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | VI secretion system | es_ES |
dc.subject | crystal structure | es_ES |
dc.subject | structural insights | es_ES |
dc.subject | nucleotide sequence | es_ES |
dc.subject | RHS proteins | es_ES |
dc.subject | colicin-B | es_ES |
dc.subject | channel | es_ES |
dc.subject | gene | es_ES |
dc.subject | requires | es_ES |
dc.subject | immunity | es_ES |
dc.title | The P. aeruginosa effector Tse5 forms membrane pores disrupting the membrane potential of intoxicated bacteria | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s42003-022-04140-y | es_ES |
dc.identifier.doi | 10.1038/s42003-022-04140-y | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoes | Inmunología, microbiología y parasitología | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | es_ES |
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