dc.contributor.author | Sot, Jesús | |
dc.contributor.author | Gartzia Rivero, Leire | |
dc.contributor.author | Bañuelos Prieto, Jorge | |
dc.contributor.author | Goñi Urcelay, Félix María | |
dc.contributor.author | Alonso Izquierdo, Alicia | |
dc.date.accessioned | 2022-12-02T18:46:44Z | |
dc.date.available | 2022-12-02T18:46:44Z | |
dc.date.issued | 2022-10 | |
dc.identifier.citation | Journal of Molecular Liquids 363 : (2022) // Article ID 119874 | es_ES |
dc.identifier.issn | 0167-7322 | |
dc.identifier.issn | 1873-3166 | |
dc.identifier.uri | http://hdl.handle.net/10810/58668 | |
dc.description.abstract | The basic matrix of cellular membranes consists of a double layer (bilayer) of phospholipids. Semisynthetic lipid bilayers are commonly used in biophysical studies of membranes. According to tem-perature and composition, lipid bilayers can exist in liquid-crystalline (or liquid-disordered), liquid -ordered, rippled, and gel phases. In the present study, the hydrophobic, solvatochromic molecule Nile red has been used as a fluorescent probe to examine the physical state of bilayers of different composi-tions in the 15-60 degrees C range. Phospholipids with saturated or unsaturated acyl chains, in the presence or absence of cholesterol have been studied. Nile red shows absorption maxima at 520-550 nm and emis-sion maxima at 580-640 nm, single photon excitation not being damaging to the system. A red/orange intensity ratio (ROIR) index has been used to normalize the results. ROIR varies clearly and reproducibly with the lipid phase, increasing in the order: liquid-ordered < gel < rippled < liquid-crystalline. It increases with temperature and decreases with cholesterol contents in the bilayers. Nile red allows an unusually clear observation of the rippled-to-liquid crystalline phase transition in saturated phospho-lipids. FLIM studies with Nile red also show differences between lamellar phases. Rotational relaxation times have been determined for Nile red in liquid-disordered (0.72 +/- 0.010 ns), gel (1.16 +/- 0.070 ns), and liquid-ordered (1.79 +/- 0.14 ns) phases, the large value of the liquid-ordered phase being an indication of the sterol hindering probe tumbling in the hydrophobic matrix. | es_ES |
dc.description.sponsorship | This work was supported in part by the Spanish Ministerio de Ciencia e Innovación (MCI), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) (grants No. PGC2018-099857-B-I00, PID2020-114755GB-C33 ), by the Basque Government (grants No. IT1625-22, IT1639-22,and IT1270-19), by Fundación Ramón Areces (CIVP20A6619), by Fundación Biofísica Bizkaia, and by the Basque Excellence Research Centre (BERC) program of the Basque Government. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICIU/PGC2018-099857-B-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2020-114755GB-C33 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | lipid bilayer phases | es_ES |
dc.subject | nile red | es_ES |
dc.subject | phospholipid rippled phases | es_ES |
dc.subject | FLIM | es_ES |
dc.subject | fluorescence | es_ES |
dc.title | Liquid-crystalline, liquid-ordered, rippled and gel lipid bilayer phases as observed with nile red fluorescence | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Authors. Published by Elsevier B.V.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S016773222201412X?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.molliq.2022.119874 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoes | Química física | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
dc.departamentoeu | Kimika fisikoa | es_ES |