Development and evaluation of different electroactive poly(vinylidene fluoride) architectures for endothelial cell culture
dc.contributor.author | Durán Rey, David | |
dc.contributor.author | Brito Pereira, Ricardo | |
dc.contributor.author | Ribeiro, Clarisse | |
dc.contributor.author | Ribeiro, Sylvie | |
dc.contributor.author | Sánchez Margallo, Juan Alberto | |
dc.contributor.author | Crisóstomo, Verónica | |
dc.contributor.author | Irastorza Epelde, Igor | |
dc.contributor.author | Silván, Unai | |
dc.contributor.author | Lanceros Méndez, Senentxu | |
dc.contributor.author | Sánchez Margallo, Francisco Miguel | |
dc.date.accessioned | 2022-12-14T17:43:16Z | |
dc.date.available | 2022-12-14T17:43:16Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Frontiers in Bioengineering and Biotechnology 10 : (2022) // Article ID 1044667 | es_ES |
dc.identifier.issn | 2296-4185 | |
dc.identifier.uri | http://hdl.handle.net/10810/58808 | |
dc.description.abstract | Tissue engineering (TE) aims to develop structures that improve or even replace the biological functions of tissues and organs. Mechanical properties, physical-chemical characteristics, biocompatibility, and biological performance of the materials are essential factors for their applicability in TE. Poly(vinylidene fluoride) (PVDF) is a thermoplastic polymer that exhibits good mechanical properties, high biocompatibility and excellent thermal properties. However, PVDF structuring, and the corresponding processing methods used for its preparation are known to significantly influence these characteristics. In this study, doctor blade, salt-leaching, and electrospinning processing methods were used to produce PVDF-based structures in the form of films, porous membranes, and fiber scaffolds, respectively. These PVDF scaffolds were subjected to a variety of characterizations and analyses, including physicochemical analysis, contact angle measurement, cytotoxicity assessment and cell proliferation. All prepared PVDF scaffolds are characterized by a mechanical response typical of ductile materials. PVDF films displayed mostly vibration modes for the a-phase, while the remaining PVDF samples were characterized by a higher content of electroactive beta-phase due the low temperature solvent evaporation during processing. No significant variations have been observed between the different PVDF membranes with respect to the melting transition. In addition, all analysed PVDF samples present a hydrophobic behavior. On the other hand, cytotoxicity assays confirm that cell viability is maintained independently of the architecture and processing method. Finally, all the PVDF samples promote human umbilical vein endothelial cells (HUVECs) proliferation, being higher on the PVDF film and electrospun randomly-oriented membranes. These findings demonstrated the importance of PVDF topography on HUVEC behavior, which can be used for the design of vascular implants. | es_ES |
dc.description.sponsorship | This work has been partially funded by the Junta de Extremadura (Spain), the Spanish Ministry of Science and Innovation, the European Social Fund, the European Regional Development Fund, and the European Next Generation Funds (Grant Numbers PD18077, TA18023, and GR21201). The authors also thanks to Portuguese Foundation for Science and Technology (FCT) for financial support under grants SFRH/BD/140698/2018 (RP), 2020.04163. CEECIND (CR). The also authors acknowledge funding by Spanish State Research Agency (AEI) and the European Regional Development Fund (ERFD) through the project PID 2019-106099RB-C43/AEI/10.13039/501100011033 and from the Basque Government Industry Departments under the ELKARTEK program. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/ PID2019-106099RB-C43 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | PVDF | es_ES |
dc.subject | films | es_ES |
dc.subject | membranes | es_ES |
dc.subject | electrospinning | es_ES |
dc.subject | tissue engineering | es_ES |
dc.subject | scaffolds | es_ES |
dc.title | Development and evaluation of different electroactive poly(vinylidene fluoride) architectures for endothelial cell culture | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 Durán-Rey, Brito-Pereira, Ribeiro, Ribeiro, Sánchez-Margallo, Crisóstomo, Irastorza, Silván, Lanceros-Méndez and Sánchez-Margallo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1044667 | es_ES |
dc.identifier.doi | 10.3389/fbioe.2022.1044667 | |
dc.departamentoes | Biología celular e histología | es_ES |
dc.departamentoeu | Zelulen biologia eta histologia | es_ES |
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Except where otherwise noted, this item's license is described as © 2022 Durán-Rey, Brito-Pereira, Ribeiro, Ribeiro, Sánchez-Margallo, Crisóstomo, Irastorza, Silván, Lanceros-Méndez and Sánchez-Margallo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.