Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
dc.contributor.author | Martín Souto, Leire | |
dc.contributor.author | Antorán Díaz, Aitziber | |
dc.contributor.author | Areitio Beramendi, Maialen | |
dc.contributor.author | Aparicio Fernández, Leire | |
dc.contributor.author | Martín Gómez, María Teresa | |
dc.contributor.author | Fernández, Roberto | |
dc.contributor.author | Astigarraga Arribas, Egoitz | |
dc.contributor.author | Barreda Gómez, Gabriel | |
dc.contributor.author | Schwarz, Carsten | |
dc.contributor.author | Rickerts, Volker | |
dc.contributor.author | Hernando Echevarria, Fernando Luis | |
dc.contributor.author | Rementeria Ruiz, Aitor Domingo | |
dc.contributor.author | Buldain Garriz, Idoia | |
dc.contributor.author | Ramírez García, Andoni | |
dc.date.accessioned | 2023-03-01T13:12:00Z | |
dc.date.available | 2023-03-01T13:12:00Z | |
dc.date.issued | 2023-01-24 | |
dc.identifier.citation | Journal of Fungi 9(2) : (2023) // Article ID 158 | es_ES |
dc.identifier.issn | 2309-608X | |
dc.identifier.uri | http://hdl.handle.net/10810/60198 | |
dc.description.abstract | The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium/Lomentospora in patients with CF. | es_ES |
dc.description.sponsorship | This research was funded by the Basque Government, grant numbers IT1362-19 and IT1657-22. L.M-S and M.A have received a predoctoral grant from the Basque Government and L.A-F from the University of the Basque Country (UPV/EHU). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Scedosporium | es_ES |
dc.subject | Lomentospora | es_ES |
dc.subject | cystic fibrosis | es_ES |
dc.subject | immunoassay | es_ES |
dc.subject | serological detection | es_ES |
dc.subject | antibody detection | es_ES |
dc.title | Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2023-02-24T14:08:25Z | |
dc.rights.holder | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2309-608X/9/2/158 | es_ES |
dc.identifier.doi | 10.3390/jof9020158 | |
dc.departamentoes | Inmunología, microbiología y parasitología | |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia |
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Except where otherwise noted, this item's license is described as © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).